脑心通胶囊对血管性痴呆大鼠CGRP的影响

目的:探讨脑心通胶囊对血管性痴呆(vascular dementia,VD)大鼠学习记忆障碍的作用及对降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的影响.方法:采用大脑中动脉梗塞法制成VD模型,将造模成功的大鼠随机分为脑心通组(以下简称中药组)、西药组和模型组,另设假手术组和正常组,进行相关治疗后以Morris水迷宫进行行为学检测,定量测定其学习记忆成绩;用Nissl染色观察其细胞形态的变化;用免疫组织化学法检测CGRP表达水平的变化.结果:中药复方脑心通胶囊可以改善VD大鼠学习记忆能力,防止海马CA1区锥体细胞死亡,同时能增加海马区域CGRP阳性细胞数和降低其灰度值.其大鼠海马CA1区内的CGRP免疫阳性细胞数明显多于模型组(P＜0.05).结论:脑心通胶囊能提高海马CGRP阳性神经元数目,对VD大鼠海马神经元起保护作用,这可能是其改善VD大鼠学习记忆能力的部分作用机制.

Effect of Naoxintong capsules on calcitonin gene-related peptideof vascular dementia in rats

OBJECTIVE: To explore the effect of Naoxintong capsules on learning and dysmnesy and calcitonin gene-related peptide (CGRP) about vascular dementia (VD) in rats. METHODS: The model of vascular dementia rats were built by middle cerebral artery occlusion (MCAO), and then the rats were randomly divided into a Naoxintong group (that was traditional Chinese medicine group,TCM group), a Western medicine group, a model group, a sham operation group, and a normal group. After corresponding treatment, the ratso behaviors were detected by Morris water maze experiment to obtain their performance record of learning and memory. The changes of cell morphology were detected by Nissl stain. The expression of CGRP in the hippocampus was measured with immunohistochemical staining. RESULTS: The masculine cells about CGRP of the TCM group were more than those of the model one (P<0.05). Naoxintong capsules improved the ability of learning and memory in vascular dementia rats, prevented the pyramidal cells on CA1 field in hippocampus from deceasing, increased the number of masculine cells about CGRP, and deceased the gray scale value. Conclusion: Naoxintong capsules can raise the number of masculine nerve cells of CGRP, protect the pyramidal cells of VD rats, and lessen their learing and dysmnesy, which may be part of the mechanism of improving learning and memory capacity of VD model in rats.