EphA2对结肠癌细胞株HCT116中VEGF和MMP9蛋白表达的影响

目的:探讨EphA2对结肠癌细胞株HCT116中血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶9(matrix metalloproteinases 9, MMP9)蛋白表达的影响.方法:用Western 印迹验证选择高表达EphA2蛋白的结肠癌细胞株HCT116;脂质体转染法将EphA2反义寡核苷酸瞬时转染入HCT116细胞,Western 印迹检测反义寡核苷酸转染效率后, ELISA检测转染前后细胞上清液中VEGF蛋白的表达;明胶酶谱分析法检测转染前后细胞上清液中明胶酶的分泌.结果:EphA2 反义寡核苷酸成功抑制了EphA2总蛋白的表达, 降低了VEGF蛋白和MMP9蛋白的表达.结论:EphA2 反义寡核苷酸通过降低结肠癌细胞株HCT116中VEGF,MMP9蛋白的表达来抑制结肠癌细胞株HCT116的侵袭和转移.

Effect of EphA2 protein on the expression of VEGF and MMP9proteins in HCT116 cells

Objective To determine the effect of EphA2 protein on the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP9) proteins in HCT116 cells. Methods High expression of EphA2 protein in HCT116 cells was confirmed by Western blot. HCT116 cells were transfected with EphA2 antisense oligonucleotide. The expression of the transfection efficiency was analyzed by Western blot. VEGF proteins in the cell supernatants were detected by enzyme linked immunosorbent assay(ELISA), and the expressions of MMP9 in cell supernatants were examined by gelatin zymography. Results EphA2 antisense oligonucleotide suppressed the expression of VEGF and MMP9 proteins in HCT116 cells. Conclusion EphA2 could decrease the invasion and metastasis of HCT116 cells by suppressing the expression of VEGF and MMP9.