心肌营养素-1在阿霉素心衰大鼠心室重构中的作用及伊贝沙坦对其的干预

目的:探讨血管紧张素Ⅱ受体1拮抗剂伊贝沙坦对阿霉素心衰大鼠心室肌中心肌营养素-1(CT-1)的表达以及对心室重构的影响.方法:30只雄性SD大鼠,随机分成2组:正常对照组(n=10)和心衰模型组(n=20).后者给予腹腔注射阿霉素造模,造模后将存活的18只大鼠随机分为模型对照组和治疗组,分别给予生理盐水(2mL/d, Qd)和伊贝沙坦(50mg/kg, Qd)灌胃.同时正常对照组也给予生理盐水(2mL/d, Qd)灌胃.灌胃12周后用免疫组织化学的方法检测CT-1的表达.结果:与正常对照组相比,模型对照组和治疗组大鼠CT-1的表达均明显升高,模型对照组大鼠心室肌CT-1和左心室质量指数呈明显正相关,治疗组的CT-1较模型对照组明显降低.结论:CT-1的过度激活在阿霉素心力衰竭大鼠的心室重构中发挥着重要作用,伊贝沙坦逆转心室重构的作用可能与其下调CT-1的表达有关.

Expression of cardiotrophin-1 and effects of irbesartan inadriamycin-induced cardiomyopathy in rats

OBJECTIVE: To investigate cardiotrophin-1 (CT-1) expression in the ventricle and the effects of angiotensin II type I receptor antagonist (AT(1)RA) irbesartan on the ventricular remodeling in adriamycin myocardiopathy. METHODS: Thirty male SD rats were randomized into 2 groups: a control group (n=10) and a model group (n=20). The model group was administered adriamycin and 18 rats survived. And theses rats were randomized again into 2 groups. One was treated with irbesartan [50 mg/(kg x d), with stomach-tube], and the other received equal saline, so did the control group. After 12 weeks, the protein level of CT-1 was detected by immunohistochemistry. RESULTS: Ventricular CT-1 in the model control group and the treatment group was higher than that in the control groupìand the correlation analysis showed that ventricular CT-1 of the model control group was positively correlated with the left ventricular weight index, and CT-1 of the treatment group was lower than that of the model control group. CONCLUSION: CT-1 was assumed to take part in the ventricular remodeling. The mechanism of irbesartan on the ventricular remodeling may be related to the downregulation of CT-1 expression.