乌司它丁对油酸致急性肺损伤大鼠肺组织血红素氧化酶-1的影响

目的:探讨乌司他丁对油酸致急性肺损伤大鼠肺组织血红素氧化酶-1表达的影响.方法 30只成年SD大鼠,随机分为3组(n＝10).正常组(A组)经大鼠尾静脉注射0.2mL/kg生理盐水;急性肺损伤组(B组)、乌司他丁组(C组)经大鼠尾静脉注射油酸0.2mL/kg制成急性肺损伤模型,随后立即给予A组、B组腹腔注射生理盐水10mL/kg,C组100ku/kg的乌司他丁腹腔注射.测取动物4h时间点呼吸频率和左心室动脉血氧分压,并于4h后取右下肺组织行HE染色和HO-1免疫组织化学检查,观察光镜下病理改变以及血红素氧化酶-1的表达.结果: 乌司他丁能显著改善油酸所致急性肺损伤所致的呼吸窘迫;肺组织血红素氧化酶-1在对照组仅有少量表达,急性肺损伤组表达增多,乌司他丁干预组表达明显增强.结论:乌司他丁可通过增加肺组织中血红素氧化酶-1的表达而发挥保护作用.

Effect of ulinastain on the expression of hemeoxygenase-1in oleic acid-induced acute lung injury in rats

Objective To explore the effect of ulinastain on the expression of hemeoxy genase-1 (HO-1) in oil acid-induced acute lung injury in rats. Methods The animal model of acute lung injury was established by oil acid. Thirty SD rats were randomly divided into 3 groups: the blank control group (A), the acute lung injury group (B) and the acute lung injury group (C) followed by injecting 100 ku/kg ulinastatin. Each group consisted of 10 rats. Group A were given 0.2 mL/kg natural solution through the trial vein; Group B and C were given 0.2 mL/kg oil-acid through trial vein, while group C were injected 100 ku/kg ulinastatin by the peritoneal cavity after injecting oil acid. After 4 hours, the rates of respiration were counted and blood samples were cramped out through the heart puncture for blood gas analysis. The expressions of hemeoxygenase-1 and the pathologic construction changes were determined by HE staining in the lower right lung of rats in the 3 groups. Results The respiration dysfunction caused by oil acid could be prominently improved by ulinastain. There was only a little expression of hemeoxygenase-1 in the lung of Group A, but the expression increased in Group B and significatively increased in Group C. Conclusion Ulinastatin may protect the rats from acute lung injury through increasing the expression of HO-1.