通脉汤对动物动脉粥样硬化的影响及其作用机制

目的:探讨动脉粥样硬化(AS)的氧化应激状态以及通脉汤对活性氧的影响.方法:日本大耳白兔40只,随机分为4组:每组10只,正常对照组以普通饲料喂养;模型组以高胆固醇饲料喂养;通脉汤低剂量治疗组以高胆固醇饲料,同时予低剂量通脉汤灌胃;通脉汤高剂量治疗组高胆固醇饲料喂养,同时予高剂量通脉汤灌胃,共喂养16周后,分别测定血清超氧化物歧化酶(SOD)活性,丙二醛(MDA)及血脂水平.结果:模型组血清SOD较正常对照组显著降低(P＜0.01),而血清MDA,CHO及LDL均显著高于对照组(P＜0.01);通脉汤低剂量治疗组血清SOD,MDA与模型组之间无显著差异(P＞0.05),血清CHO及LDL较模型组显著降低(P＜0.05);通脉汤高剂量治疗组血清SOD较模型组显著升高(P＜0.05),MDA,CHO及LDL均较模型组显著下降(P＜0.01).相关分析结果表明动脉粥样硬化时血清MDA与CHO,LDL成显著正相关(分别r=0.397,0.443,P＜0.05);SOD与CHO及LDL成显著负相关(分别r=-0.407,-0.429,P＜0.01).结论:通脉汤能降低血清CHO,LDL,升高SOD水平,在动脉粥样硬化中起抗氧化的作用.

Effect and mechanism of Tongmai Tang in atherosclerosis

Objective To study the changes of reactive oxygen species(ROS), and the effect of Tongmai Tang on ROS. Methods Forty rabbits were randomly divided into 4 groups: a normal control group,a model group, a low dosage Tongmai Tang, and a high dosage Tongmai Tang administration group (n=10).The normal control group was fed with common food twice daily. The model group was fed with high cholesterol diet. The low dosage Tongmai Tang administration group was fed with high cholesterol diet as in the model group, together with Tongmai Tang at a lower dosage. The high dosage Tongmai Tang administration group was fed with high cholesterol diet as in the model group, together with Tongmai Tang at a higher dosage. After 16 weeks, the concentration of serum malondialdehyde (MDA),superoxide dismutase(SOD), and serum lipid were detected. Results In the model group, the concentration of serum SOD was significantly lower, while levels of serum MDA,CHO, and LDL were significantly higher than those in the control group(P<0.01). In the low dosage Tongmai Tang administration group, the concentration of serum CHO and LDL significantly decreased (P<0.05). In the high dosage Tongmai Tang administration group, the concentration of serum SOD significantly increased (P<0.05), whereas the level of serum MDA, CHO and LDL significantly decreased (P<0.01). MDA was positively correlated with serum CHO, LDL (r= 0.397 and 0.443, P<0.05), and SOD was negetively correlated with serum CHO, LDL(r=-0.407 and -0.429, P<0.01). Conclusion Tongmai Tang can decrease the concentration of serum CHO and LDL, and increase the level of SOD. Tongmai Tang may have antioxidation in atherosclerosis.