Radiometric Determination of the Concentration of Amphotericin B in Body Fluids |
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| Authors: | Roy L Hopfer and Dieter Grschel |
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| Institution: | Department of Laboratory Medicine, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77030 |
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| Abstract: | A new assay was developed to determine the concentration of amphotericin B in body fluids. The Bactec radiometric system was used to measure CO2 production by a test strain, Candida albicans MDA 448, in the presence of amphotericin B. After 5-h incubation, drug concentrations as low as 0.2 μg/ml could be detected. The results are comparable to those of the commonly used agar diffusion assay with Paecilomyces varioti. |
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