| 长时连续作业对大鼠下丘脑及背缝核orexin-A和受体的影响 |
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| 引用本文： | 王臻,谌小维,张春青,樊宏孝,程赛宇,阮怀珍,胡志安.长时连续作业对大鼠下丘脑及背缝核orexin-A和受体的影响[J].第三军医大学学报,2006,28(10):1046-1048. |
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| 作者姓名： | 王臻 谌小维 张春青 樊宏孝 程赛宇 阮怀珍 胡志安 |
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| 作者单位： | 第三军医大学基础医学部,神经生物学教研室,重庆市神经科学研究所,重庆,400038;第三军医大学基础医学部,基础医学教学实验中心,重庆,400038 |
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| 基金项目： | 第三军医大学军事医学创新预研基金资助项目(XJYY200310)~~ |
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| 摘 要： | 目的研究长时连续作业对大鼠促觉醒肽orexin- A及受体表达的影响。方法使用长时连续作业动物模型,采用免疫组织化学方法结合图像分析技术测定orexin -A及受体OX1R在下丘脑外侧区,背缝核的变化。结果经过长时连续作业训练后,跑台组与对照组相比,orexin- A阳性细胞在下丘脑外侧区、orexin- A阳性纤维在背缝核及OX1R在背缝核均显著减少。结论长时连续作业可造成促觉醒肽orexin- A和OX1R的表达降低。
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| 关 键 词： | 长时连续作业 orexin-A 疲劳 觉醒 免疫组织化学 |
| 文章编号： | 1000-5404（2006）10-1046-03 |
| 收稿时间： | 2005-11-24 |
| 修稿时间： | 2006-01-05 |
Effects of long-term continuous operations on orexin-A and OX1R expression in the rat brain |
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| WANG Zhen,CHEN Xiao-wei,ZHANG Chun-qing,FAN Hong-xiao,CHENG Sai-yu,RUAN Huai-zhen,HU Zhi-an.Effects of long-term continuous operations on orexin-A and OX1R expression in the rat brain[J].Acta Academiae Medicinae Militaris Tertiae,2006,28(10):1046-1048. |
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| Authors: | WANG Zhen CHEN Xiao-wei ZHANG Chun-qing FAN Hong-xiao CHENG Sai-yu RUAN Huai-zhen HU Zhi-an |
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| Institution: | 1.Department of Neurobiology, 2.Experimental Center of Basic Medicine, College of Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective To investigate the effects of long-term continuous operations on orexin-A and its receptor (OX1R) expression in the lateral hypothalamic area (LHA) and dorsal raphe nucleus (DR) of a rat model. Methods Rats exercised for two circadian cycles using a treadmill protocol. Subsequently, immunohistochemical techniques combined with quantitative analysis of images techniques were performed to detect the expression of orexin-A and OX1R in rat LHA and DR. Results The number of orexin-A immunoreactive neurons in LHA, orexin-A immunoreactive fibers and OX1R in DR of operational animals was significantly decreased as compared with control group (P<0.01). Conclusion Rat alertness activities are deleteriously influenced by long-term continuous operations, which may be related to the depressed expression of orexin-A and its receptor in the arousal systems. |
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| Keywords: | long-term continuous operations orexin-A fatigue wakefulness immunohistochemistry |
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