| 微板法检测蚊虫体内非特异性酯酶活性研究 |
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| 引用本文： | 赵玉强,李士根,李文,甄天民,闫歌,王新国,孙传红,王怀位,刘凤梅.微板法检测蚊虫体内非特异性酯酶活性研究[J].医学动物防制,2001,17(3):121-123. |
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| 作者姓名： | 赵玉强 李士根 李文 甄天民 闫歌 王新国 孙传红 王怀位 刘凤梅 |
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| 作者单位： | 山东省寄生虫病防治研究所,272033 |
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| 基金项目： | 山东省卫生厅,医科院资助项目 |
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| 摘 要： | 目的 探讨蚊虫抗药性敏感测试方法,方法 采用微板法,对室内常规饲养的敏感品系和室内选育的DDVP、抗残杀威品系淡色库蚊非特异性酯酶的活性进行了测定。结果 敏感品系蚊虫OD值≥0.9的占17.16％(35/204条);<0.9的占82.84％(169/204条);抗DDVP品系OD值≥0.9的占83.33％(200/240条);<0.9的占16.67％(40/240条);抗残杀威品系≥0.9的占74.89％(176/235条),<0.9的占25.11％(59/235条)。结论 敏感品系与抗性品系淡色库蚊的平均OD值存在较大差异。
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| 关 键 词： | 微板法 蚊虫 非特异性酯酶 |
Microplate Test for the Determination of Non-specific Esterase in Vector Mosquitoes |
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| Zhao Yu-Qiang Li Shi-Gen Li Wen et al.Microplate Test for the Determination of Non-specific Esterase in Vector Mosquitoes[J].Chinese Journal of Pest Control,2001,17(3):121-123. |
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| Authors: | Zhao Yu-Qiang Li Shi-Gen Li Wen |
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| Abstract: | Microplate assay was employed for the determination of nonspecific esterase level in individual susceptible, anti-DDVP and anti-propoxur resistant Culex pipiens. Among susceptible mosquitoes detected, 17. 16% was of high esterase activity with OD value more than 0. 9. Among anti-DDVP and antipropoxur resistant mosquitoes, the OD value more than 0. 9 was found in 83. 33% and 74. 89% respectively. This indicated that there existed obvious difference in the level of nonspecific esterase between susceptible and resistant mosquitoes. |
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| Keywords: | Microplate Mosquito Non-specific esterase Insecticide resistance |
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