针灸对小鼠骨髓细胞微核的影响

目的 :研究针灸对小鼠骨髓细胞微核的影响。方法 :将实验动物分为正常对照组 ,阳性对照Ⅰ、Ⅱ组 ,针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ、Ⅱ组。针刺小鼠“足三里” ,艾灸“关元”穴 ,检测小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的变化。结果 :阳性对照组与正常对照组比较 ,小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率明显升高。针灸预防Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,针灸治疗Ⅰ组与阳性对照Ⅰ组比较 ,针灸治疗Ⅱ组与阳性对照Ⅱ组比较 ,各指标明显降低 (P <0 0 0 1)。结论 :针灸可拮抗由环磷酰胺诱导的小鼠骨髓嗜多染红细胞微核率、骨髓有核细胞微核率的升高

Effect of Acupuncture and Moxibustion on Micronucleus Formation of Polychromatic Erythroblasts and Nucleated Cells in Mouse Bone Marrow

Objective: To observe the effect of acupuncture and moxibustion (acumoxi) on micronucleus formation of bone marrow polychromatic erythroblasts and nucleated cells.Methods:72 Kunming mice were randomly and evenly divided into normal control group, positive control groupⅠ and Ⅱ; prevention groupⅠ, Ⅱ, Ⅲ and Ⅳ; and treatment groupⅠand Ⅱ.In the later 8 groups, all the mice were given (i.p.) with cyclophosphamide (CP, 50 mg/kg) for inducing micronucleus formation, after one day (1 d)'s, 2 ds', 4ds' and 10 ds' treatment in prevention groupⅠ, Ⅱ, Ⅲ and Ⅳ; 24 hr and 48 hr before killing the rats in model group Ⅰ and Ⅱ; and before treatment in treatment groupⅠand Ⅱ. Bilateral “Zusanli"(ST 36) and “Guanyuan” (CV 4) were punctured first, followed by moxibustion for 3 min, once daily. At the end of each experiment, all the mice were decapitated, and micronucleus incidence of the bone marrow polychromatic erythroblasts (10,000/group) and nucleated cells (10,000/group) were observed and calculated under light microscope. Results:Compared with normal control group, the incidences of micronuclei of polychromatic erythroblasts and nucleated cells of model group Ⅰ and Ⅱ were significantly higher (P<0.001), suggesting that CP could cause the bone marrow cells to generate mutation. In comparison with model group Ⅰ and Ⅱ, the incidences of micronuclei of these two types of cells in prevention group Ⅰ, Ⅱ, Ⅲ and Ⅳ and treatment group Ⅰ were significantly lower (P<0.001). While in comparison with model group Ⅱ, the incidences of micronuclei of these two types of cells in treatment group Ⅱ were also considerably lower (P< 0.001). It showed that acupuncture and moxibustion work well in relieving and preventing CP induced mutation of the bone marrow cells in mice. Conclusion:Acupuncture and moxibustion of “Zusanli"(ST 36) and “Guanyuan"(CV 4) can prevent and relieve intraperitoneal administration of CP induced micronucleus formation of bone marrow cells in the mouse.