| 载TK基因聚丙交酯乙交酯纳米粒的制备及有关性质研究 |
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| 引用本文： | 何勤,张志荣,刘戟,苏敏,张华,卢懿,聂飞,罗鹏. 载TK基因聚丙交酯乙交酯纳米粒的制备及有关性质研究[J]. 生物医学工程学杂志, 2002, 19(1): 30-33 |
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| 作者姓名： | 何勤 张志荣 刘戟 苏敏 张华 卢懿 聂飞 罗鹏 |
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| 作者单位： | 1. 四川大学,华西药学院,成都,610041
2. 四川大学,生物化学与分子生物学研究所,重组DNA研究室,成都,610041 |
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| 摘 要： | 我们构建了含AFP启动子、TK基因和EGFP真核表达载体重组的质粒，并以可生物降解、生物相容的高分子载体材料PLGA包埋制成载自杀基因的纳米粒。结果表明纳米粒形态圆整，大小均匀，平均粒径68nm，包封率可达80%，该纳米粒能显著提高质粒DNA抵抗核酸酶降解和超声波剪切的能力。
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| 关 键 词： | 自杀基因 聚丙交酯乙交酯 纳米粒 包封率 |
Study of Encapsulation of Suicide Gene in Poly(D,L-lactic-co-glycolic acid) Nanoparticles and Its Characteristics in vitro |
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| He Qin Zhang Zhirong Liu Ji Shu Min Zhang Hua Lu Yi Nie Fei Luo Peng. Study of Encapsulation of Suicide Gene in Poly(D,L-lactic-co-glycolic acid) Nanoparticles and Its Characteristics in vitro[J]. Journal of biomedical engineering, 2002, 19(1): 30-33 |
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| Authors: | He Qin Zhang Zhirong Liu Ji Shu Min Zhang Hua Lu Yi Nie Fei Luo Peng |
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| Affiliation: | School of Pharmacy, Sichuan University, Chengdu 610041. |
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| Abstract: | We constructed the AFP promotor, suicide gene and EGFP eukaryotic expression vector recombinant plasmid , and this plasmid DNA was encapsulated by biodegradable , biocompatible polymer PLGA to prepare nanoparticles. The results demonstrate that the mean diameter of DNA PLGA NP is 68nm, the encapsulation ratio reaches to 80%,and the PLGA nanoparticles can protect plasmid DNA from digestion by deoxyribonuclease I (DNaseI) and sonication induced shearing in vitro. |
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| Keywords: | Suicide gene Poly(D L lactic co glycolic acid) Nanoparticles Encapsulation ratio |
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