转基因西洋参冠瘿组织中人参皂苷类成分的分离鉴定

 目的分离鉴定转基因西洋参冠瘿组织中的化学成分。方法以本研究组转导和培养的西洋参冠瘿组织为实验材料,体积分数为70%乙醇超声提取,硅胶柱色谱分离,用MS,¹H-NMR、¹³C-NMR、HMBC等谱学方法确定结构。结果分离得到5个化合物,分别为20(S)-人参皂苷Rh₁(Ⅰ)、20(S)-人参皂苷Rg₃(Ⅱ)、人参皂苷Re(Ⅲ)、20(S)-达玛烷-3β,6α,12β,20,25-五醇-6-O-α-L-鼠李吡喃糖基-(1→2)-O-β-D-吡喃葡萄糖苷(Ⅳ)和人参皂苷Rg₁(Ⅴ)。结论上述5个成分均为首次从转基因西洋参冠瘿组织培养物中分离得到,化合物Ⅳ为新天然产物。

Study on Isolation and Structural Elucidation of Ginsenosides from Transgenic Crown Gall of Panax quinquefolium

OBJECTIVE To isolate and identify ginsenosides from transgenic crown gall cultures of Panax quinquefolium. METHODS The chromatography on silica gel was used for the isolation and purification of constituents.The structures were elucida- ted by MS,¹H-NMR,¹³C-NMR and HMBC spectral analysis.RESULTS Five ginsenosides were obtained and identified as follows: 20(S)-ginsenoside Rh₁(Ⅰ),20(S)-ginsenoside Rg₃(Ⅱ),ginsenoside Re(Ⅲ),20(S)-dammaran-3β,6α,12β,20,25-pentol-6-O-α-L-rhampyranoyl-(1→2)-O-β-D-glucopyranoside(Ⅳ)and ginsenoside Rg₁(Ⅴ).CONCLUSION All five ginsenosides were firstly isolated from the transgenic crown gall cultures of Panax quinquefolium and compoundⅣwas a new natural product.