VEGF—C反义多肽对乳腺癌裸鼠移植瘤影响的实验研究

目的观察血管内皮生长因子C（VEGF—C）反义多肽对乳腺癌裸鼠皮下移植瘤的影响，探讨以VEGF—C反义多肽用于乳腺癌基因治疗的应用前景。方法制备靶向VEGF—C的反义多肽，构建乳腺癌细胞MCF-7裸鼠皮下移植瘤模型，瘤体内注射VEGF—C反义多肽，观察其对乳腺癌细胞的抑制作用；免疫印迹（Western Blot）检测VEGF—C在瘤组织的表达情况。结果VEGF—C反义多肽具有较强的生物学活性，瘤组织内给予VEGF-C反义多肽后，Western blot分析VEGF—C在瘤组织中的表达降低，肿瘤生长受制，抑瘤率为52％（P〈0．05）。结论 制备出的VEGF—C具有良好的生物学活性，有抗乳腺癌作用。

Anti-tumor effect of the antisense peptide of VEGF-C on human breast cancer cells in a murine xenograft model

Objective To investigate the anti - tumor effect of the antisense peptide of vascular endothelial growth factor - C （ VEGF - C） on human breast cancer cells in a routine xenograft model Methods The model of subcutaneous tumor was generated by injection of human breast cancer cells MCF - 7 into the nude mice, antisense peptide of VEGF - C was subcutaneous injected around xenograft tumors after tumor cell innoculation. Tumor growth was observed, VEGF - C expression in tumor tissues was measured by western blot. Results Western blot analysis showed VEGF - C protein was weakly expressed in tumor tissue ; tumor growth was significantly inhibited in mice injected with antisense peptide of VEGF - C, and the tumor growth - inhibiting rate was 52% （ P 〈 0.05 ）. Conclusion Anfisense pepfide of VEGF - C exhibits effective biological activity and inhibits tumor growth.