| HLA-G5-IgGFc融合蛋白真核表达载体的克隆及鉴定 |
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| 引用本文： | 陈庆,吴雄文,杨敬宁,梁智辉. HLA-G5-IgGFc融合蛋白真核表达载体的克隆及鉴定[J]. 华中医学杂志, 2005, 29(1): 3-4 |
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| 作者姓名： | 陈庆 吴雄文 杨敬宁 梁智辉 |
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| 作者单位： | 430030,武汉,华中科技大学同济医学院免疫教研室;430030,武汉,华中科技大学同济医学院免疫教研室;430030,武汉,华中科技大学同济医学院免疫教研室;430030,武汉,华中科技大学同济医学院免疫教研室 |
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| 基金项目： | 国家自然科学基金资助项目(No.30271201)，科技部973计划资助课题(No.2001CB510008) |
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| 摘 要： | 目的构建人HLA-G5-IgGFc融合蛋白真核表达载体。方法经重组PCR将HLA-G5和IgGFc基因拼接并插入T载体。鉴定后，双酶切得到融合基因并插入真核表达载体pcDNA3.1。结果PCR、限制性内切酶酶切和序列分析表明已构建pcDNA3.1-HLA-G5表达载体。结论成功构建了HLA-G5-IgGFc段融合蛋白真核表达载体。
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| 关 键 词： | DNA 重组 基因融合 基因 MHC Ⅰ类 免疫球蛋白类 Fc |
| 修稿时间： | 2004-09-03 |
Clone and construction of eukaryotic expression vector HLA-G5-IgGFc fusion protein gene |
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| Chen Qing,Wu Xiongwen,Yang Jingning et al. Clone and construction of eukaryotic expression vector HLA-G5-IgGFc fusion protein gene[J]. Central China Medical Journal, 2005, 29(1): 3-4 |
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| Authors: | Chen Qing Wu Xiongwen Yang Jingning et al |
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| Affiliation: | Chen Qing,Wu Xiongwen,Yang Jingning et al. Department of Immunology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
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| Abstract: | Objective To construct the eukaryotic expression vector of HLA-G5-IgGFc fusion protein. Methods The DNA fragments of HLA-G5 and those of IgGFc were linked together through overlapping PCR. The product was inserted in T vector. Obtaining by double restriction endonucleases digestion, the objective gene fragment was inserted into the eukaryotic expression vector pcDNA 3.1 after it having been identified.Results By PCR, restriction endonucleases treatment and sequencing, the construction of recombination plasmid was confirmed to be successful.Conclusion The eukaryotic expression vector pcDNA 3.1 -HLA-G5 was successfully constructed. |
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