| 高效液相色谱法测定青霉素V钾片中主药的含量 |
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| 引用本文： | 金建平. 高效液相色谱法测定青霉素V钾片中主药的含量[J]. 中国药房, 2005, 16(17): 1338-1339 |
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| 作者姓名： | 金建平 |
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| 作者单位： | 安徽芜湖市药品检验所,芜湖市,241000 |
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| 摘 要： | 目的:建立以高效液相色谱法测定青霉素V钾片中主药含量的方法。方法:色谱柱为DiamonsilC18,流动相为乙腈-0.02mol/L醋酸钠(70∶30),流速为1.0ml/min,检测波长为268nm,灵敏度为0.01AUFS,柱温为(26±1)℃,进样量为20μl。结果:青霉素V钾检测浓度在10.0～120.0μg/ml范围内与峰面积呈良好的线性关系(r=0.9999,n=5);平均加样回收率为100.2%(RSD=0.42%)。结论:本方法快速、简便、准确,可用于青霉素V钾片的质量控制。
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| 关 键 词： | 高效液相色谱法 青霉素V钾 含量 |
| 文章编号： | 1001-0408（2005）17-1338-02 |
| 收稿时间： | 2004-10-17 |
| 修稿时间： | 2005-03-04 |
Content Determination of the Principal Agent in Phenoxymethylpenicillin Potassium Tablets by HPLC |
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| Jin Jianping. Content Determination of the Principal Agent in Phenoxymethylpenicillin Potassium Tablets by HPLC[J]. China Pharmacy, 2005, 16(17): 1338-1339 |
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| Authors: | Jin Jianping |
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| Abstract: | OBJECTIVE:To establish HPLC method for the determination of the principal agent in phenoxymethylpenicillin potassium tablets.METHODS:The determination was performed on Diamonsil C 18 column with column temperature at(26±1)℃.The mobile phase consisted of acetonitrile-0.02mol/L sodium acetate(70∶30)with a flow rate at1.0ml/min.The detection wavelength was set at268nm;the sensitivity was0.01AUFS and the sample size was20μl.RESULTS:Good linear correlation with peak area was noted when the detection concentration range was10.0～120.0μg/mL for phenoxymethylpeniˉcillin potassium(r=0.9999,n=5).The average recovery was100.2%(RSD=0.42%).CONCLUSION:The method was rapid,simple and accurate and it can be used to determine the content of the principal agent in phenoxymethylpenicillin potasˉsium tablets. |
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| Keywords: | HPLC Phenoxymethylpenicillin potassium Content |
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