海南地区女性HLA-DQB1和DRB5基因多态性与宫颈癌易感性的关系

目的 探讨海南地区汉族女性人群中人类白细胞抗原(HLA)-DQB1和HLA-DRB5等位基因多态性与宫颈癌易感性的相关性.方法 以海南地区41例经病理确诊的宫颈癌患者和38例因子宫肌瘤行全子宫切除术且病理证实无宫颈癌的患者为研究对象.采用PCR克隆测序技术对两组HLA-DQB1和DRB5等位基因进行分型.结果 共检测到12种HLA-DQB1等位基因和8种HLA-DRB5等位基因.其中HLA-DQB1*06011,HLA-DQB1* 030101和HLA-DQB1* 03032与HLA-DRB5* 01:01:01分别是海南地区汉族女性HLA-DQB1和DRB5的优势表达基因.无宫颈癌患者HLA-DRB5*0206等位基因频率(18.4％)显著高于宫颈癌患者(0)(P=0.013).结论 HLA-DRB5* 0206可能是海南地区女性宫颈癌的保护基因,可能降低女性患宫颈癌的风险.HLA-DRB5不同等位基因可能与宫颈癌的发生存在相关性.

Association between polymorphisms of human leukocyte antigen HLA-DQB1 and HLA-DRB5 alleles and susceptibility to cervical cancer in Hainan province

Objective To investigate the association between polymorphism of human leukocyte antigen (HLA-DQB1 and HLA-DRB5) alleles and susceptibility to cervical cancer.Methods 41 patients with cervical cancer and 38controls were selected in Hainan Han population as study objectives.PCR and gene sequencing analysis were used to genotype HLA-DQB1 and HLA-DRB5.Results A total of 12 HLA-DQB1 alleles and 8 HLA-DRB5 alleles were detected in both group.The frequencies of HLA-DQB1 * 06011,HLA-DQB1 * 030101,HLA-DQB1 * 03032 and HLA -DRB5 * 01:01:01 were the commonest alleles in Han female population in Hainan province.In this study,HLA-DRB5 * 02:06 allele was significantly higher in controls (18.4％) compared with patients with cervical cancer (0％,P =0.013).Conclusion HLA-DRB5 * 0206 may be a protective factor from cervical cancer among Hainan Han women,implying that polymorphic amino acids at HLA-DRB5 allele may play an important role in the development of cervical cancer.