SIRT1对肺癌A549细胞增殖和迁移的影响

目的 通过调节肺癌A549细胞中沉默信息调节因子1(SIRT1)的表达,探讨SIRT1对肺癌细胞增殖和转移的影响及其分子机制.方法 培养肺癌A549细胞,用重组腺病毒感染A549细胞上调/下调SIRT1的表达;通过MTT实验、克隆形成实验、划痕实验和Transwell迁移实验检测上调/下调SIRT1表达对肺癌A549细胞增殖和转移的影响;干扰SIRT1表达后用Western blot检测SIRT1对肺癌A549细胞中上皮细胞向间质细胞转化(EMT)相关蛋白标记物ZO-1、β-连环蛋白(β-catenin)、Snail和ZEB1表达的影响.结果 上调/下调SIRT1表达对肺癌A549细胞增殖无明显影响,但是可以促进/抑制肺癌A549细胞迁移;下调SIRT1表达后EMT相关上皮性标记蛋白ZO-1表达增多,间质性标记蛋白β-catenin、Snail和ZEB1表达减少.结论 SIRT1可能通过影响EMT相关标记蛋白的表达促进肺癌A549细胞迁移.

SIRT1 promotes the migration of lung cancer cells A549 through influencing EMT

Objective To investigate the influence of SIRT1 on cell proliferation and migration in A549 cells. Methods lung cancer A549 cells were culture .The recombinant adenovirus was applied to up -regulate/down-regu-late the expression of SIRT1.MTT assay, colony formation, wound-healing assay and trans -well migration assay were performed to measure multiplication capacity and migration ability .Western Blot assay was performed to detect the influ-ence of down -regulating SIRT1 on the expression of EMT related proteins , including ZO -1, β-catenin, Snail and ZEB1.Results After up -regulating/down -regulating the expression of SIRT1, the proliferation rate of A549 cell showed no obvious change , but healing rate and migration ability of A 549 cells were enhanced/weakened .The level of EMT related epithelial markers ZO -1 was increased, while the level of interstitial markers β-catenin, Snail and ZEB1 were decreased after down -regulating the expression of SIRT1.Conclusion SIRT1 promotes lung cancer cell A549 mi-gration through influencing EMT related proteins .