CC16在脓毒症大鼠心肌中的表达

目的 观察Clara细胞分泌蛋白(Clara cell secretory 16-KD protein,CC16)在脓毒症大鼠心肌组织中的表达,探讨其在脓毒症心肌损伤发生机制中的作用.方法 将40只健康雄性Wistar大鼠随机分为假手术组(SH组,n=20)及脓毒症组(CLP组,n=20),采用盲肠结扎穿孔法(CLP)制备脓毒症动物模型,24 h后,检测各组的血清脑钠肽(BNP)、肌钙蛋白I(cTnI)的水平,观察心肌组织病理变化及心肌组织CC16的表达水平.结果 CLP组出现明显的心肌损伤,CLP组血清BNP、cTnI高于SH组(P<0.01),心肌CC16低于SH组(P<0.01).结论 CC16在脓毒症大鼠心肌组织中表达下降,CC16可能在脓毒症心肌损伤发病机制中起重要作用.

Expression of CC16 in myocardial tissues in septic rats.

Objective To investigate the expression of CC 16 in myocardial tissues in septic rats , and its role in sepsis-induced myocardial injury in rats .Methods Forty healthy male Wistar rats were randomly divided into sham group (SH group, n=20) and sepsis group (CLP group, n=20).The sepsis models were established by cecal ligation and puncture (CLP).The levels of Serum brain natriuretic peptide (BNP) and cardiac troponin I (cTnI) were measured 24 hours after CLP .The myocardial tissue pathological changes and the expression of CC 16 in myocardial tissue were ob-served.Results In CLP group, myocardial tissues were seriously injured , the levels of Serum BNP and cTnI were signif-icantly higher than SH group (P<0.01), and the level of myocardial CC16 was significantly lower than SH group (P<0.01).Conclusion The expression of CC16 decreases in myocardial tissues in septic rats , CC16 may play an important role in the pathogenesis of sepsis -induced myocardial injury .