| 重组人癌胚抗原cDNA基因转染小鼠肝癌细胞体外实验模型的建立 |
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| 引用本文： | 卢方安,黄冰,等.重组人癌胚抗原cDNA基因转染小鼠肝癌细胞体外实验模型的建立[J].郧阳医学院学报,1999,18(4):191-193. |
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| 作者姓名： | 卢方安 黄冰 |
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| 作者单位： | 郧阳医学院生化教研室!十堰，442000，中山医科大学实验动物中心，中山医科大学生化教研室，中山医科大学生化教研室，中山医科大学生化教研室，中山医科大学生化教研室 |
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| 摘 要： | 目的：建立表达人癌胚抗原（CEA）的小鼠肝癌细胞模型。方法：用脂质体介导转染法将含人CEA-cDNA的逆转录病毒重组质粒pLXSN-CEA导入小鼠肝癌细胞，利用含G418的选择培养基筛选及放免法测定癌细胞CEA表达量进行鉴定。结果：成功建立表达人CEA的小鼠肝癌细胞模型。结论：利用基因转染技术能将人癌胚抗原cDNA基因导入小鼠肝癌细胞中，并能在选择培养基中生长，且表达人的癌胚抗原。
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| 关 键 词： | 癌胚抗原 基因 肝肿瘤 小鼠 转染 |
The Establishment of An in Vitro Experimental Model of Murine Hepa Cancer cells Transferred by Recombinant Human CEA Plasmid |
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| Lu Fangan, Huang Bing,Yang Jie et al.The Establishment of An in Vitro Experimental Model of Murine Hepa Cancer cells Transferred by Recombinant Human CEA Plasmid[J].Journal of Yunyang Medical College,1999,18(4):191-193. |
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| Authors: | Lu Fangan Huang Bing Yang Jie |
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| Abstract: | Objective:To establish murine Hepa cancer cell model expressing human carcinoembryonic antigen (CEA).Methods: The recombinant plasmid pLXSN-CEA with human CEAswhd pcoN - CEA with human CEA-cDNA was transferred into the ng lipofecedne. FuIther screening with G4l8 and edng of CEA expressed by the Hepa cancer cells with radio-immunology assay were taken to identify the CEA positive caner cells.Results: The Hepa caner cells model of murine expressing human CEA was obtained successfully. Conclusion:Human CEA - cDNA plasmid could be transferred into murine Hepa cancer cells by DNA transfer techniques. |
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| Keywords: | Carcinoembryonic Antigen Genes Liver Neoplasms Mice Transfection |
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