| 大鼠骨髓间质干细胞体外分化为成骨细胞的实验研究 |
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| 引用本文： | 孙晓春,许文荣,朱伟,胡嘉波,陈巧林,钱晖,王波.大鼠骨髓间质干细胞体外分化为成骨细胞的实验研究[J].江苏大学学报(医学版),2004,14(5):369-372. |
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| 作者姓名： | 孙晓春 许文荣 朱伟 胡嘉波 陈巧林 钱晖 王波 |
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| 作者单位： | 1. 江苏大学医学技术学院,江苏,镇江,212001
2. 江苏大学附属医院,江苏,镇江,212001 |
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| 基金项目： | 江苏省自然科学基金资助项目 (BK2 0 0 2 0 0 6)，江苏省高校自然科学基金资助项目 ( 0 3KJD3 10 0 63、0 2KJB3 10 0 0 2 ) |
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| 摘 要： | 目的：建立大鼠骨髓间质干细胞(mesenchymal stem cells，MSCs)体外诱导分化为成骨细胞的模型。方法：分离大鼠骨髓间质干细胞进行体外培养，观察其生物学特性。并选用一定的诱导剂诱导成骨细胞，通过形态学变化、碱性磷酸酶染色及钙沉积对成骨细胞进行鉴定。结果：大鼠MSCs细胞形态呈长梭形，成骨细胞诱导后MSC细胞形态由长梭形向多边形转变，ALP染色阳性，Von Kossa染色阳性，呈现典型的成骨细胞形态和生物学特征。结论：大鼠骨髓MSCs体外能被诱导分化为成骨细胞，为骨组织工程研究的优良种子细胞。
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| 关 键 词： | 大鼠 骨髓间质干细胞 成骨细胞 诱导 |
| 文章编号： | 1671-7783(2004)05-0369-03 |
| 修稿时间： | 2004年9月6日 |
An Experimental Study on Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in Vitro |
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| SUN Xiao-chun,XU Wen-rong,ZHU Wei,HU Jia-bo,CHEN Qiao-li n,QIAN Hui,WANG Bo.An Experimental Study on Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells in Vitro[J].Journal of Jiangsu University Medicine Edition,2004,14(5):369-372. |
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| Authors: | SUN Xiao-chun XU Wen-rong ZHU Wei HU Jia-bo CHEN Qiao-li n QIAN Hui WANG Bo |
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| Institution: | SUN Xiao-chun1,XU Wen-rong1,ZHU Wei1,HU Jia-bo1,CHEN Qiao-li n1,QIAN Hui1,WANG Bo3 |
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| Abstract: | Objective: To established a model of rat bone ma rr ow mesenchymal stem cells differentiated into osteoblast in vitro. Method s: MSCs were isolated and cultured in vitro. Investigated the basic biol ogical characteristics. The MSCs was induced for differentiate of osteoblast und er the conditional culture media. Differentiation into the osteoblast were docum ented by observed the change of morphology, deposition of calcium and visualizat ion of alkaline phosphatase activity. Results: Rat MSCs had spind le shape, and changed to polygonal appearance after being induced. Cytochemistry evaluation showed that MSCs were positive for ALP and Von Kossa stain was posit ive. Conclusion: The MSCs can differentiated into osteoblasts lin eage in vitro. MSCs can be served as optimal cell source for study on osteogenic tissue engineering. |
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| Keywords: | Rat MSCs Osteoblast Induction |
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