| Bmi-1 siRNA对人白血病K562细胞增殖的影响及其机制 |
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| 引用本文： | 朱勇,胡义德,胡中华. Bmi-1 siRNA对人白血病K562细胞增殖的影响及其机制[J]. 第三军医大学学报, 2008, 30(9): 791-794 |
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| 作者姓名： | 朱勇 胡义德 胡中华 |
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| 作者单位： | 第三军医大学新桥医院全军肿瘤诊治研究所,重庆,400037;第三军医大学新桥医院全军肿瘤诊治研究所,重庆,400037;第三军医大学新桥医院全军肿瘤诊治研究所,重庆,400037 |
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| 基金项目： | 国家自然科学基金(30772144) |
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| 摘 要： | 目的探讨Bmi-1 siRNA对人白血病K562细胞增殖的影响及其作用机制。方法通过脂质体将化学合成针对Bmi-1基因的siRNA转染K562细胞，采用MTT法观察Bmi-1 siRNA对K562细胞增殖的抑制作用；流式细胞术分析细胞周期变化；Western blot检测Bmi-1 siRNA对K562细胞中Bmi-1和P16蛋白表达的影响。结果Bmi-1 siRNA能明显延长K562细胞的倍增时间、G1期比例增加；下调Bmi-1表达的同时上调P16蛋白表达。结论体外化学合成的Bmi-1 siRNA对K562细胞的体外增殖具有明显的抑制作用，下调Bmi-1蛋白表达的同时上调P16蛋白表达。
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| 关 键 词： | Bmi-1基因 小分子干扰RNA 人白血病K562细胞 P16蛋白 |
| 文章编号： | 1000-5404(2008)09-0791-04 |
| 修稿时间： | 2007-12-29 |
RNAi-silenced human Bmi-1 gene inhibits proliferation of human leukemia cell line K562 |
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| ZHU Yong,HU Yi-de,HU Zhong-hua. RNAi-silenced human Bmi-1 gene inhibits proliferation of human leukemia cell line K562[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(9): 791-794 |
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| Authors: | ZHU Yong HU Yi-de HU Zhong-hua |
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| Abstract: | Objective To explore the silence of B-cell specific Moloney murine leukaemia virus insertion site 1(Bmi-1)by RNA interference on the proliferation of human leukemia cell line K562 and its mechanisms.Methods Small interfering RNA(siRNA)targeting Bmi-1 gene was designed and double-stranded siRNA was chemically synthesized.After double-stranded siRNA was transfected into K562 cells with Lipofectamine 2000,the proliferation of K562 cells was detected by MTT colorimetry,cell cycle was determined by flow cytometr... |
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| Keywords: | B-cell specific Moloney murine leukaemia virus insertion site-1 gene small interference RNA human leukemia cell line K562 P16 protein |
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