抗人大肠癌免疫磁性白蛋白微球的抗癌效果

目的:观察自制丝裂霉素C免疫磁性白蛋白微球(MMC-IMAMS)体外及体内抗大肠癌效果。方法:将丝裂霉素C免疫磁性白蛋白微球(MMC-IMAMS)与丝裂霉素C磁性白蛋白微球(MMC-MAMS)、单纯丝裂霉素C(MMC)比较,MTT法分别检测其体外对大肠癌细胞株LoVo的杀伤作用,然后通过移植人大肠癌裸鼠模型检测体内的肿瘤抑制率。结果:体外实验丝裂霉素免疫磁性白蛋白微球较单纯丝裂霉素磁性白蛋白微球有更强的肿瘤细胞抑制效率;裸鼠模型体内实验丝裂霉素免疫磁性白蛋白微球组肿瘤抑制率较丝裂霉素磁性白蛋白微球组和单纯丝裂霉素组具有显著差异。且丝裂霉素免疫磁性白蛋白微球是安全的,在体外磁场作用下可较长时间内沉积于靶区。结论:丝裂霉素免疫磁性白蛋白微球安全性好,靶向性更强大,体内体外均有更强的抗癌效果。

Anticancer effects of mitomycin loaded immunomagnetic albumin microspheres against human colorectal cancer

Objective:To evaluate the killing effect of mitomycin loaded immunomagnetic albumin microspheres(MMC-IMAMS) against human colorectal cancer in vitro and in vivo.Methods:using MTT assay to evaluate the specific cytotoxic effects on target LoVo cells of MMC-IMAMS,mitomycin loaded magnetic albumin microspheres(MMC-MAMS),and mitomycin(MMC) in vitro.Then MMC-IMAMS,MMC-MAMS,and MMC were injected into nude mices bearing human colorectal carcinoma respectively to study the inhibitory activity in vivo.Results:The cytotoxic effects of MMC-IMAMS group were higher than MMC-MAMS group and MMC group.The inhibitory rate of MMC-IMAMS group on the growth of colorectal cancer xenografts was significantly higher than that of MMC-MAMS group or MMC group.MMC-IMAMS were safe and could deposit at the target area for quite a long time under the influence of magnetic field.Conclusion:The MMC-IMAMS are safe and have higher targeting and anticancer ability than MMC-MAMS in vitro and vivo.