| 脐带间质干细胞心肌样分化中干细胞标志物的表达 |
| |
| 引用本文： | 钱茜,徐哲,史云燕,沈俐,孙靖,张徐,毛飞,朱伟,钱晖,许文荣.脐带间质干细胞心肌样分化中干细胞标志物的表达[J].江苏大学学报(医学版),2011,21(3):185-189. |
| |
| 作者姓名： | 钱茜 徐哲 史云燕 沈俐 孙靖 张徐 毛飞 朱伟 钱晖 许文荣 |
| |
| 作者单位： | 江苏大学基础医学与医学技术学院,江苏,镇江,212013 |
| |
| 基金项目： | 国家自然科学基金资助项目，江苏省自然科学基金资助项目，江苏省重大成果转化项目，江苏省科技支撑计划，江苏大学科技创新团队和拔尖人才项目，江苏大学大学生科研立项 |
| |
| 摘 要： | 目的:探讨人脐带间质干细胞 (human umbilical cord mesenchymal stem cells,hucMSCs)在体外特定条件下是否可以定向分化为心肌样细胞及干细胞标志物的表达变化.方法:采用脐带组织块贴壁培养方法分离人脐带间质干细胞,用第2代hucMSC进行体外心肌细胞诱导分化;采用 RT-PC...
|
| 关 键 词： | 人脐带间质干细胞 心肌样细胞 分化 干细胞标志物 |
Expression of stemness markers in the cardiac differentiation derived from human umbilical cord mesenchymal stem cells |
| |
| QIAN Qian,XU Zhe,SHI Yun-yan,SHEN Li,SUN Jing,ZHANG Xu,MAO Fei,ZHU Wei,QIAN Hui,XU Wen-rong.Expression of stemness markers in the cardiac differentiation derived from human umbilical cord mesenchymal stem cells[J].Journal of Jiangsu University Medicine Edition,2011,21(3):185-189. |
| |
| Authors: | QIAN Qian XU Zhe SHI Yun-yan SHEN Li SUN Jing ZHANG Xu MAO Fei ZHU Wei QIAN Hui XU Wen-rong |
| |
| Institution: | QIAN Qian,XU Zhe,SHI Yun-yan,SHEN Li,SUN Jing,ZHANG Xu,MAO Fei,ZHU Wei,QIAN Hui,XU Wen-rong(School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
| |
| Abstract: | Objective: To investigate whether hucMSCs could differentiate into cardiomyocyte phenotype in vitro and explain the expression of stem cell markers.Methods: hucMSCs were isolated from human umbilical cord.The hucMSCs from passage 2 were selected for differentiation to cardiomyocytes in vitro.The differentiated cells were identified by RT-PCR and transmission electron microscope.Techniques of flow cytometry,real-time PCR and western blot were used to analyze the expression of stem cell-associated genes and p... |
| |
| Keywords: | hucMSCs cardiomyocyte phenotype differentiation stem cell markers |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《江苏大学学报(医学版)》浏览原始摘要信息 |
| 点击此处可从《江苏大学学报(医学版)》下载免费的PDF全文 |
|
