| 黑龙江立克次体外膜蛋白B基因表达及表达重组蛋白的抗原性分析 |
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| 引用本文： | 孟艳芬,段长松,王锡乐,熊小路,温博海. 黑龙江立克次体外膜蛋白B基因表达及表达重组蛋白的抗原性分析[J]. 寄生虫与医学昆虫学报, 2011, 18(1): 28-33. DOI: 10.3969/j.issn.1005-0507.2011.01.006 |
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| 作者姓名： | 孟艳芬 段长松 王锡乐 熊小路 温博海 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071 |
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| 基金项目： | 国家基础研究计划项目(973计划课题)，国家科技重大专项(重大传染病预防与控制课题) |
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| 摘 要： | 黑龙江立克次体为远东蜱传斑点热病原体,本文以黑龙江立克次体基因组为模板,扩增ompB的4段基因,利用原核表达载体构建重组表达质粒,IPTG诱导重组质粒转化的大肠杆菌表达重组目的蛋白,并对其抗原性进行分析.免疫印迹反应表明,重组表达的4个蛋白均与黑龙江立克次体感染小鼠血清反应.该4个重组蛋白分别免疫小鼠后,分析其血清的特...
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| 关 键 词： | 黑龙江立克次体 外膜蛋白 抗原性 |
Antigenicity Analysis of Recombinant Outer Membrane Protein B of Rickettsia heilongjiangensis |
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| MENG Yan-Fen,DUAN Chang-Song,WANG Xi-Le,XIONG Xiao-Lu,WEN Bo-Hai. Antigenicity Analysis of Recombinant Outer Membrane Protein B of Rickettsia heilongjiangensis[J]. Acta Parasitologica et Medica Entomologica Sinica, 2011, 18(1): 28-33. DOI: 10.3969/j.issn.1005-0507.2011.01.006 |
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| Authors: | MENG Yan-Fen DUAN Chang-Song WANG Xi-Le XIONG Xiao-Lu WEN Bo-Hai |
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| Affiliation: | (State Key Laboratory of Pathogens and Biosecurity, Belting Institute of Microbiology and Epidemiology, Beijing 100071 , China ) |
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| Abstract: | Rickettsia heilongjiangensis is a human pathogen that causes Far East tick-borne spotted fever. Four fragments of outer membrane protein B gene （ompB） were amplified from R. heilongjiangensis, and the gene fragments were recombined with prokaryotic expression plasmid. Four recombinant OmpB proteins were expressed in E. coli cells transformed with the recombinant plasmids, respectively, following IPTG stimulation. In immunoblot assay, the recombinant OmpB proteins reacted with sera from mice infected with R. heilongfiangensis. In immunofluorescence assay, the sera from mice immunized with each recombinant OmpB protein strongly reacted with R. heilongjiangensis and the specific antibody titers were≥ 1：5 120, and the prominent cross-reactivity was observed between the immune sera and R. rickettsii （ ≥1：40） while the little cross-reactivity was observed between the immune sera and typhus group rickettsiae （ ≤1 ： 40）. The results suggest that the recombinant OmpB proteins have a good antigenicity since they have capability to efficiently elicit humoral immunoresponses and to specifically react with rickettsial antibodies. |
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| Keywords: | Rickettsia heilongjiangensis Outer membrane protein Antigenicity |
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