| Chemerin重组腺病毒表达载体的构建 |
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| 引用本文： | 王慧,张迪,黄剑,杨键,袁国跃,杨颖. Chemerin重组腺病毒表达载体的构建[J]. 江苏大学学报(医学版), 2011, 21(3): 241-244,248 |
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| 作者姓名： | 王慧 张迪 黄剑 杨键 袁国跃 杨颖 |
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| 作者单位： | 1. 上海市内分泌代谢病研究所,上海,200025
2. 江苏大学附属医院内分泌代谢科,江苏,镇江,212001 |
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| 基金项目： | 国家自然科学基金资助项目，镇江市社会发展项目 |
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| 摘 要： | 目的:应用基因重组技术,构建表达chemerin基因的重组腺病毒载体.方法:经PCR方法扩增得到chemerin基因,将其插入到带有绿色荧光蛋白(green fluorescent protein,GFP)基因的穿梭载体pShuttle-IRES-hrGFP-1中,得到重组质粒pShuttle-chemerin-IRE...
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| 关 键 词： | 重组腺病毒载体 chemerin 非乙醇性脂肪肝 |
Construction of recombinant adenovirus containing chemerin gene |
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| WANG Hui,ZHANG Di,HUANG Jian,YANG Jian,YUAN Guo-yue,YANG Ying. Construction of recombinant adenovirus containing chemerin gene[J]. Journal of Jiangsu University Medicine Edition, 2011, 21(3): 241-244,248 |
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| Authors: | WANG Hui ZHANG Di HUANG Jian YANG Jian YUAN Guo-yue YANG Ying |
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| Affiliation: | WANG Hui1,ZHANG Di1,HUANG Jian1,YANG Jian1,YUAN Guo-yue2,YANG Ying1(1.Shanghai Institute of Endocrine and Metabolic Diseases,Shanghai 200025,2.Department of Endocrinology,the Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001,China) |
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| Abstract: | Objective: Application of recombinant DNA technology to construct recombinant adenovirus vector express chemerin.Methods: Chemerin gene was amplified by PCR method,clone the gene of interest into the shuttle vector pShuttle-IRES-hrGFP-1 with green fluorescent protein gene(GFP) using appropriate sites in the MCS.Made linearized pShuttle-chemerin-IRES-hrGFP-1 into the BJ5183 competent cells containing the plasmid pAdEasy-1 for homologous recombination.Recombinant adenovirus Ad-chemerin was linearized by the r... |
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| Keywords: | chemerin |
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