人隐孢子虫IbA19G2基因亚型蒙古沙鼠感染模型的建立

人隐孢子虫Cryptosporidium hominis是人类的主要感染虫株,目前已获取且传代保存的国内分离株较少,在生物学特性研究也未见报道.在本试验自感染前第10天起至排卵囊结束后14天,采用胃管灌服地塞米松(0.75 mg/(只·2天))抑制蒙古沙鼠免疫力,对国内C.hominis Ib亚型分离株生物学特性进行研...

Laboratory Model for Cryptosporidium hominis IbA19G2 Subtype in Mongolian Gerbils

Researches have shown that Cryptosporidium hominis is one of the main Cryptosporidium parasites for human cryptosporidiosis in China. However, only a few isolates of C. hominis were obtained and serially sub-cultivated in laboratory up to date, so the biological characteristics of C. hominis remains unreported in our country. In the present work, Mongolian gerbils （Meiiones unguiculataus） were orally inoculated with 0.75 mg of dexamethasone/animal by using gastric tube drench every two days from the 10th day before oocyst ingestion. The results showed that the gerbils started to shed oocysts in the third day after inoculation （DAI） , the number of shed oocysts increased obviously, reached the peak value at the 6th DAI, and then dropped gradually until 10th or llth DAI. The 18S rRNA and gp60 gene analysis performed before and after inoculation revealed no genetic difference of C. hominis oocysts. A stable rodent model of C. hominis Ib subtype infection has been successfully established, which may be an important value for the further study on the biological characteristics of C. hominis.