柞蚕雄蛾提取液改善W256荷瘤大鼠放疗后免疫功能的实验研究

摘要：目的观察柞蚕雄蛾提取液对荷W256癌肉瘤大鼠放疗后免疫功能的影响。方法60只Wistar大鼠随机分为正常对照组、模型组、单纯放疗组和实验组（放疗与柞蚕蛾提取液联合治疗组），每组15只。建立W256荷瘤大鼠模型，分离脾脏和胸腺，并计算脏器指数；采用流式细胞术（FCM）及酶联免疫吸附试验（ELISA）分别检测外周血T淋巴细胞亚群变化和血清中细胞因子INF-γ和IL-4含量。结果与放疗组相比较，柞蚕雄蛾提取液可以升高放疗后荷瘤大鼠脾脏和胸腺指数，增加放疗后荷瘤大鼠外周血中CD4⁺T细胞比例与CD4⁺T/CD8⁺T值（P＜0.05）；实验组荷瘤大鼠血清中细胞因子INF-γ含量明显升高，而IL-4含量显著降低（P＜0.05）。结论柞蚕雄蛾提取液可以逆转荷瘤大鼠放疗后免疫功能抑制状态，改变荷瘤机体免疫功能，可作为恶性肿瘤传统放疗中的辅助用药。

Effect of an extract from the male zooid of Antheraea pernyi on reversing radiation-induced immunosuppression in rats bearing W256 carcinoma

Abstract: ObjectiveTo study the effects of an extract from the male zooid on reversing the radiation-induced immunosuppression of rats bearing W256 carcinoma. MethodsTumor models were established by subcutaneously injecting W256 tumor cells into rats. 60 Wistar rats were randomly divided into 4 groups. The thymus index and the spleen index were used as parameters to evaluate the effect of an extract from the male zooid on non-specific and specific immune function of rats with tumor. The presence of Interferon gamma (INF-γ) and interleukin-4 (IL-4) in the peripheral blood serum were screened by enzyme-linked immunosorbent assay (ELISA) to investigate cell-mediated immune responses and T subsets were determined by flow cytometry(FCM). ResultsThe extract from the male zooid significantly raised the thymus index and the spleen index of rats with tumors. The number of CD4⁺T and the CD4⁺T/CD8⁺T ratio of W256-bearing rats treated with the extract from the male zooid significantly increased compared with the radiotherapy group (P＜0.05). Also, the level of Interferon-gamma (INF-γ) was up-regulated and interleukin-4(IL-4) was down-regulated. ConclusionsThe extract from the male zooid of Antheraea pernyi has effect of reversing the radiation-induced immunosuppression and could be regarded as a main assistant agent combined with traditional radiotherapy for treatment of malignant tumors.