华蟾素注射液联合氟尿嘧啶对大肠癌Lovo细胞的凋亡作用

目的 研究华蟾素注射液联合5-氟尿嘧啶（5-Fu）对大肠癌Lovo细胞的凋亡抑制。方法 实验分四组：空白对照组、单独使用5-Fu组、单独使用华蟾素组、5-Fu联合华蟾素组（5-Fu+华蟾素组）,通过四甲基偶氮唑盐（MTT）比色法检测细胞的增殖抑制,Annexin V-FITC /PI流式细胞双染法检测细胞凋亡,流式细胞术分析细胞周期。结果 通过MTT比色法得出,作用24 h,Lovo细胞抑制率：5-Fu+华蟾素组=华蟾素组>5-Fu组>对照组（P>0.05）,作用48 h,Lovo细胞抑制率：5-Fu+华蟾素组>华蟾素组>5-Fu组>对照组,（P<0.05）,提示5-Fu+华蟾素组对Lovo细胞有明显的增殖抑制作用（P<0.05）,但抑制率和时间无明显对应关系（P>0.05）。Annexin V-FITC /PI法根据早、晚期凋亡率及总凋亡率比较,5-Fu +华蟾素组>5-Fu组>华蟾素组>对照组（P<0.05）,得出5-Fu+华蟾素组可以加速Lovo细胞凋亡。流式细胞术根据G1/S比例,5-Fu +华蟾素组>5-Fu组>华蟾素组>对照组（P<0.05）,证明5-Fu+华蟾素组可以更好抑制细胞由G1期向S期转化,减少肿瘤分裂增殖。结论 华蟾素注射液联合5-氟尿嘧啶对大肠癌Lovo细胞有更好地抑制作用,可加速凋亡,且非时间依赖型,但单用华蟾素注射液并没有比传统的5-Fu效果更好。

Inhibitory effect of cinobufacini injection combined with 5-Fu on apoptosis of Lovo cells in colorectal carcinoma

Objective To study the inhibitory effect of cinobufacini injection combined with 5-fluorouracil(5-Fu) on apoptosis of Lovo cells in colorectal carcinoma.Methods In this experiment, there were four groups: a blank control group, 5-Fu group, cinobufacini group, and 5-Fu+cinobufacini group. The cell proliferation inhibition was detected by MTT colorimetric assay, the cell apoptosis was detected by Annexin V-FITC /PI Flow cytometry, and the cell cycle was analyzed by the flow cytometry. Results The MTT colorimetric assay showed that the effect of the drug(s) for 24 h, the Lovo cell inhibition rate presented 5-Fu+cinobufacini group = cinobufacini group >5-Fu group > control group (P>0.05); for 48h, the Lovo cell inhibition rate presented the 5-Fu+cinobufacini group >cinobufacini group >5-Fu group > control group(P<0.05). The above mentioned results meant that 5-Fu+cinobufacini group had a significant inhibitory effect on the proliferation of Lovo cells (P<0.05), but there was no significant correlation between the inhibition rate and time (P>0.05). The Annexin V-FITC /PI method showed that compared with the early and late apoptosis rates and total apoptosis rate (5-Fu+cinobufacini group >5-Fu group >cinobufacini group > control group, P<0.05),the combined use of drugs could accelerate the apoptosis of Lovo cells. The flowcytometry showed that based on the ratio of G1/S (5-Fu+cinobufacini group >5-Fu >cinobufacini group > control group, P<0.05),the 5-Fu+ cinobufacini group could inhibit the transformation of cells from phase G1 to phaseS.Conclusion Cinobufacini injection combined with 5-Fu has a better inhibitory effect on colorectal cancer Lovo cells, and it is not time dependent. However, the use of cinobufacini alone is not better than the use of traditional 5-Fu.