β-1,4-半乳糖苷键在周围神经损伤后的表达变化

为了探讨正常和损伤的周围神经中β-1,4-半乳糖苷键的表达定位和表达变化 ,本研究用特异识别β-1,4-半乳糖苷键的蓖麻凝集素 -I免疫组织化学方法结合 S-10 0和 neurofilam ent免疫双标记 ,研究了大鼠坐骨神经损伤对 β-1,4-半乳糖苷键的合成影响。结果表明 ,β-1,4-半乳糖苷键主要分布在正常和损伤坐骨神经的 Schwann细胞中 ,图像分析表明β-1,4-半乳糖苷键的表达在周围神经损伤后 1～ 2 d内有所增高 ,以后随时间延长表达量逐步下降。本研究结果提示 ,周围神经损伤对 β-1,4-半乳糖苷键的表达有影响 ,Schwann细胞表达的β-1,4-半乳糖苷键可能参与周围神经损伤修复相关因子的修饰调控

LOCALIZATIONS AND CHANGES OF Galβ-1,4-GIcNAc GROUP IN RAT LESION SCIATIC NERVES

To study the localizations and changes of Galβ-1, 4-GlcNAc group in normal and injured peripheral nerves, the expression of Galβ-1, 4-GlcNAc group in the injured sciatic nerves of rats was studied by lectin histochemistry with ricinus communis agglutinin-I (RCA-I). It was found that Galβ-1, 4-GlcNAc group was localized mainly in Schwann cells, and reached its peak within 1-2 days in both stumps of lesion nerves after axotomy, then decreased thereafter. The expression of Galβ-1, 4-GlcNAc group changed in the peripheral nerves after injury. These results suggest that Galβ-1, 4-GlcNAc group may be involved in the galactosylation of nerve regeneration associated factors which play an important role in the regeneration of injured nerves.