| 胃癌中抑癌基因FHIT外显子5纯合性的缺失与突变 |
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| 引用本文： | 陈兵,孙立峰,朱华庆,周青,汪渊,袁凌云. 胃癌中抑癌基因FHIT外显子5纯合性的缺失与突变[J]. 安徽医科大学学报, 2000, 35(6): 436-437 |
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| 作者姓名： | 陈兵 孙立峰 朱华庆 周青 汪渊 袁凌云 |
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| 作者单位： | 安徽医科大学生物化学与分子生物学教研室，合肥 230032 |
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| 摘 要： | 目的 探讨候选的抑癌基因FHIT外显子5在胃癌中纯合性缺失与突变的情况。方法 用PCR方法研究外显子5的纯合性缺失，用PCR－SSCP研究外显子5的点突变。结果 在32例原发性胃癌中外显子5的纯合性缺合率为9．38％（3／32），没发现外显子5存在点突变。结论ＦＨＩＴ外显子５的缺失在胃癌中不一定占主导地位。
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| 关 键 词： | 胃肿瘤/遗传学 基因,抑制,肿瘤 突变 |
| 文章编号： | 1000-1492(2000)06-0436-03 |
| 修稿时间： | 2000-11-15 |
Analysis of homozygous deletion and point mutation of FHIT gene exon 5 in gastric carcinoma |
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| Chen Bing,Sun Lifeng,Zhu Huaqing et al. Analysis of homozygous deletion and point mutation of FHIT gene exon 5 in gastric carcinoma[J]. Acta Universitis Medicinalis Anhui, 2000, 35(6): 436-437 |
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| Authors: | Chen Bing Sun Lifeng Zhu Huaqing et al |
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| Abstract: | Objective To investigate the homozygous deletion and point mutation of FHIT gene exon 5 gastric carcinoma. Methods PCR,PCR SSCP were used to analyze the homozygous deletion and point mutation of FHIT exon 5. Results In the 32 samples of gastric carcinoma, homozygous deletion of FHIT exon 5 was detected in 3 cases with a deletion rate of 9 4% and no point mutation of FHIT exon 5 was found in 32 cases. Conclusion The homozygous deletion and point mutation of FHIT exon 5 may not play an important role during inactivating the FHIT gene of gastric cancer. |
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| Keywords: | stomach neoplasms/genetics genes suppressor tumor mutation |
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