二仙汤对去卵巢大鼠骨质疏松的影响

目的探讨二仙汤防治去卵巢大鼠骨质疏松的作用及其初步机制。方法实验SD大鼠分为假手术组、模型对照组、尼尔雌醇组和二仙汤高、中、低剂量组等6组,除假手术组施行假手术外,其余均行手术彻底摘除卵巢,术后连续10d以阴道涂片检测动情周期筛选动物,然后依照假手术组、模型对照组灌胃生理盐水,尼尔雌醇组灌胃尼尔雌醇(1.2 mg/(kg.w)),二仙汤高、中、低剂量组分别灌胃淫羊藿提取液(剂量依次为含生药12、8、4 g/kg),连续给药90 d,考察骨密度、骨生物力学,以及血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)、丙二醛(MDA)的变化。结果与模型对照组相比,二仙汤能明显提高去卵巢大鼠骨密度(P0.01)、股骨三点弯曲最大弯应力(P0.01)和L5腰椎压缩载荷力(P0.01),提高血清ALP和SOD活性(P0.01),降低血清ACP和MDA的含量(P0.01)。结论二仙汤能明显改善去卵巢大鼠骨质疏松症,促进成骨细胞增殖分化、抑制破骨细胞功能,抗氧化应激可能是其机制之一。

Effect of Er-xian decoction on osteoporosis in ovariectomized rats

Objective To investigate the effect of Er-xian decoction on osteoporosis in ovariectomized rats and its preliminary mechanism. Methods The female SD rats were divided into 6 groups: sham operated group (Sham), ovariectomized group (Control), Nilestriol with ovariectomized group (Nilestriol), High concentration Er-xian decoction with ovariectomized group (HDED), middle concentration Er-xian decoction with ovariectomized group (MDED), and low concentration Er-xian decoction with ovariectomized group (LDED). Rats in Sham group received sham operation, while all the other rats were ovariectomized. The vaginal smear was observed to detect the estrous cycle of rats during consecutive 10 days after the operation, in order to screen rats. Then the screened rats in Sham group and the Control group were gavaged with normal saline. Rats in Nilestriol groups were gavaged with 1.2mg/kg nilestriol. The rats in HDED group, MDED gropu, and LDED group were gavaged with the extraction of herba epimedii, with the dosage of 12, 8, and 4 g/kg, respectively. All the treatment lasted for 90 days. The changes of bone mineral density (BMD), bone biomechanics characteristics, and the serum levels of ALP, ACP, SOD, and MDA were observed. Results Compared with that in the Control group, Er-xian decoction could significantly improve BMD, the maximum bending stress, and the loading force of the 5th lumbar vertebra (P<0.01), increase serum ALP and SOD activity (P<0.01), and lower the serum levels of ACP and MDA (P<0.01). Conclusion Er-xian decoction can significantly relieve symptoms of osteoporosis in ovariectomized rats, promote the proliferation and differentiation of osteoblasts, inhibit the function of osteoclasts. Antioxidant stress may be one of the possible mechanisms.