采用OB胶粘贴法建立人胃癌裸鼠原位种植转移模型

背景与目的建立理想的动物肿瘤移植模型是进行肿瘤防治研究的重要前提,目前公认的胃癌造模的方法为采用完整组织块的“皮下移植法”、“胃囊法”,但传统的造模方法目前尚存在操作复杂、成活率低等缺点,本研究拟采用“OB胶粘贴法”更简便地建立人胃癌裸鼠原位移植模型。方法以反复接种传代于裸鼠皮下的SGC鄄7901人胃癌细胞株建立的移植瘤组织块为材料,将其用生物吻合“OB胶”原位粘贴于裸鼠胃壁,并与传统“胃囊法”、“皮下移植法”比较观察移植肿瘤的生长情况、移植成功率和自发转移的发生率。结果“OB胶粘贴法”组原位移植成功率为100%,局部淋巴结转移率为100%,肺转移发生率为62.5%,肝转移发生率为87.5%,腹膜转移发生率为87.5%,较之胃囊法(分别为100%、100%、50.0%、50.0%和33.3%),除腹膜转移率升高外,远处脏器转移差异无显著性,但成活率高。皮下移植组未发生局部浸润及远处转移。结论“OB胶粘贴法"能更简便地建立人胃癌高转移模型,重现临床转移过程。

Nude mouse model of human gastric carcinoma metastasis constructed by orthotopic transplantation using organism glue paste technique

BACKGROUND & OBJECTIVE: Ideal animal model of tumor metastasis is an important precondition for the research on tumor prevention and treatment. Traditional gastric cancer models were constructed using full tumor tissue block by "subcutaneous transplantation" or "gastric bursa method" with limitations, such as operative complexity, and low survival rate. We tried to construct nude mouse model of human gastric carcinoma metastasis by orthotopic transplantation using organism glue paste technique to solve the problems. METHODS: Transplanted gastric tumor tissue block was constructed through repeatedly subcutaneously inoculating and passaging human gastric carcinoma SGC-7901 cells in nude mouse, and pasted orthotopically on the stomach wall of BALB/c nude mouse with OB biological glue. Tumor growth, successful rate of orthotopic transplantation, and incidence of metastasis in nude mice performed organism glue paste were compared with those in nude mice performed subcutaneous transplantation or gastric bursa method. RESULTS: In nude mice performed organism glue paste, the successful rate of orthotopic transplantation was 100%, the metastasis rate of local lymph node, lung, liver, and peritoneum were 100%, 62.5%, 87.5%, and 87.5%, respectively, while those in nude mice performed gastric bursa method were 100%, 100%, 50.0%, 50.0%, and 33.3%, respectively. The nude mice performed organism glue paste showed a increased metastatic rate of peritoneum (P < 0.05) with a higher survival rate. No local invasion and distance metastasis was observed in nude mice performed subcutaneous transplantation. CONCLUSION: Organism glue paste technique can create gastric cancer metastasis model more easily than traditional methods, and represent clinical metastasis process.