Antimicrobial and antibiofilm activity of LL-37 and its truncated variants against Burkholderia pseudomallei |
| |
Authors: | Kanthawong Sakawrat Bolscher Jan G M Veerman Enno C I van Marle Jan de Soet Hans J J Nazmi Kamran Wongratanacheewin Surasakdi Taweechaisupapong Suwimol |
| |
Affiliation: | a Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand b Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand c Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, 1081 BT Amsterdam, The Netherlands d Department of Electron Microscopy, Academic Medical Center, University of Amsterdam, The Netherlands e Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, 1081 BT Amsterdam, The Netherlands f Biofilm Research Group, Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand |
| |
Abstract: | The Gram-negative bacterium Burkholderia pseudomallei is the aetiological agent of melioidosis, which is an endemic disease in tropical areas of Southeast Asia and Northern Australia. Burkholderia pseudomallei has intrinsic resistance to a number of commonly used antibiotics and has also been reported to develop a biofilm. Resistance to killing by antimicrobial agents is one of the hallmarks of bacteria grown in biofilm. The aim of this study was to determine the antimicrobial activity and mechanisms of action of LL-37 and its truncated variants against B. pseudomallei both in planktonic and biofilm form, as LL-37 is an antimicrobial peptide that possessed strong killing activity against several pathogens. Antimicrobial assays revealed that LL-31, a truncated variant of LL-37 lacking the six C-terminus residues, exhibited the strongest killing effect. Time-kill experiments showed that 20 μM LL-31 can reach the bactericidal endpoint within 2 h. Freeze-fracture electron microscopy of bacterial cells demonstrated that these peptides disrupt the membrane and cause leakage of intracellular molecules leading to cell death. Moreover, LL-31 also possessed stronger bactericidal activity than ceftazidime against B. pseudomallei grown in biofilm. Thus, LL-31 should be considered as a potent antimicrobial agent against B. pseudomallei both in planktonic and biofilm form. |
| |
Keywords: | Burkholderia pseudomallei Antimicrobial peptide LL-37 Truncated variants Biofilm |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|