| HPLC 法测定兔血浆中阿西美辛和吲哚美辛的浓度 |
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| 引用本文： | 刘振华,金 英,李忠思.HPLC 法测定兔血浆中阿西美辛和吲哚美辛的浓度[J].河北医学,2014(3):353-355. |
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| 作者姓名： | 刘振华 金 英 李忠思 |
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| 作者单位： | [1] 承德医学院附属医院药剂科,河北 承德067000 [2] 承德医学院中药研究所/河北省中药研究与开发重点实验室,河北 承德067000 |
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| 基金项目： | 河北省科技厅指导性课题（编号：052761271） |
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| 摘 要： | 目的：建立家兔血浆中阿西美辛和吲哚美辛浓度的测定方法。方法：采用高效液相色谱法，以酮洛芬为内标，色谱柱：Hypersil BDS C18（4．6 mm＆#215;250mm，5μm），流动相为pH 4．5醋酸盐缓冲液-乙腈-甲醇（50∶40∶10），流速为0．6 mL/min，柱温为25℃，检测波长为250nm，进样量为20μL。结果：阿西美辛在0．04-2．56μg/mL浓度范围内线性关系良好，平均回收率为89．4％，RSD＝5．2％（ n＝5）；吲哚美辛在0．03-1．92μg/mL浓度范围内线性关系良好，平均回收率为89．7％，RSD＝5．7％（ n＝5）。结论：本法简单、灵敏、结果准确，可用于测定兔血浆中阿西美辛和吲哚美辛的浓度。
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| 关 键 词： | 阿西美辛 吲哚美辛 高效液相色谱法 |
Determination of Acemetacin and Indometacin in Rabbits Plasma by HPLC |
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| Abstract: | Objective：To establish a method for the determination of acemetacin and indometacin con-centrations in rabbits plasma .Method： HPLC method was adopted .Ketoprofen was used as the internal standard,Hypersil BDS C18 （4.6 mm＆#215;250mm,5μm） column was used,the mobile phase consisted pH4.5 acetate buffer solution-acetonitrile-methanol（50 ∶40 ∶10）,flow rate was 0.6mL/min,column temperature was 25℃,the detection wave length was 250nm,injection volume was 20μL.Result： The linear range of acemetacin was 0.04-2.56μg/mL,the average recovery was 89.4%,RSD=5.2%（n=5）;The linear range of indometacin was 0.03-1.92μg/mL,the average recovery was 89.7%,RSD=5.7%（n=5）.Conclusion：The method is simple ,sensitive and accurate , and it can be applied to the determination of acemetacin and indo-metacin concentrations in rabbits plasma . |
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| Keywords: | Acemetacin Indometacin HPLC |
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