| NKCC1基因敲除小鼠的饲养繁殖及其鉴定 |
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| 引用本文： | 吴振恭,褚汉启,熊浩,韩芳,黄孝文,崔永华. NKCC1基因敲除小鼠的饲养繁殖及其鉴定[J]. 听力学及言语疾病杂志, 2006, 14(5): 379-380,390,i0004 |
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| 作者姓名： | 吴振恭 褚汉启 熊浩 韩芳 黄孝文 崔永华 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院耳鼻咽喉科,武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目(编号30371526)，湖北省自然学基金项目(编号2002AB127) |
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| 摘 要： | 目的繁殖和鉴定NKCC1基因敲除小鼠,建立实验动物模型。方法将引进的NKCC1基因敲除小鼠饲养于相对无噪声的隔离环境中,依照遗传学规则进行繁殖及培育,提取小鼠尾部组织全基因组DNA进行聚合酶链式反应(PCR)检测各种系小鼠的基因型。结果共繁殖出220只子代小鼠,经鉴定,NKCC1 / 小鼠占32%,NKCC /-小鼠占50%,NKCC-/-小鼠占18%,其中,NKCC-/-小鼠生长较另外二型小鼠明显缓慢。结论采用Jack-on Laboratry提供的聚合酶链式反应法(PCR)可以成功地鉴别出不同基因表型的NKCC1基因敲除鼠,为研究NKCC1基因敲除小鼠相关课题提供了实验基础。
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| 关 键 词： | Na-K-2Cl联合转运子1 基因敲除 小鼠 |
| 文章编号： | 1006-7299（2006）05-0379-03 |
| 收稿时间： | 2006-03-23 |
| 修稿时间： | 2006-03-23 |
Breeding, Reproducing, and Identifying NKCC1 Knock-out Mice |
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| Wu Zhengong, Chu Hanqi, Xiong Hao,et al.. Breeding, Reproducing, and Identifying NKCC1 Knock-out Mice[J]. Journal of Audiology and Speech Pathology, 2006, 14(5): 379-380,390,i0004 |
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| Authors: | Wu Zhengong Chu Hanqi Xiong Hao et al. |
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| Affiliation: | Wu Zhengong, Chu Hanqi, Xiong Hao, et al. |
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| Abstract: | Objective To breed and identify NKCC1 (Na~ -K~ -2Cl~- cotransporter) knock-out mice and to establish an animal experimental model for study.Methods NKCC1 knock-out mice were bred and reproduced under relative noise-free feeding conditions according to Mendel law. Genome DNA extracted from the mice tails was subjected to PCR for genotype identification.Results The experimental model for NKCC1 knock-out mice was successfully established and more NKCC1 knock-out mice were reproduced.Conclusion The polymerase chain reaction experiment method that Jackon Laboratry proposes can succeed in distinguishing different genetic NKCC1 gene of phenotype. Furthermore,it can lay out a solid foundation to further study NKCC1 knock-out mice correlation topic. |
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| Keywords: | NKCC1 Knock-out Mice |
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