Different doses of partial liver irradiation promotes hepatic regeneration in rat |
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| Authors: | Ying Liu Changzheng Shi Meng Cui Zhenhua Yang Danhui Gan Yiming Wang |
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| Affiliation: | 1.Department of Oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;2.Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China;3.Department of Pathology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China |
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| Abstract: | The aim of this study is to investigate whether partial liver irradiation promotes hepatic regeneration in rat. Left-half liver of rat was irradiated to 10 Gy, and the Right-half to 0, 5, 10 and 15 Gy, respectively. Then, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) levels were evaluated on 0 day, 15-day, 30-day, 45-day and 60-day after liver irradiation. Next, the serum HGF, NF-κB and TGF-β1 levels were also analyzed on 60-day after liver irradiation. Lastly, the cyclinD1 protein expression was appraised by western blots on 60-day after liver irradiation. ALT, AST and ALP levels were reduced compared with that of controls. The serum HGF, NF-κB and TGF-β1 levels, and the cyclinD1 protein expression in liver irradiation group were increased compared with that of controls group. However, hepatic regeneration of higher dose-irradiated cirrhotic liver was triggered a more enhanced regeneration, compared with that of higher doses group. In summary, these results suggest that different doses of partial liver irradiation promotes hepatic regeneration in rat. |
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| Keywords: | Liver irradiation, hepatic regeneration, HGF, NF-κ B, TGF-β 1 |
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