MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon |
| |
| Authors: | Xiyu Duan Haijun Li Zhen Qiu Bishnu P Joshi Asha Pant Arlene Smith Katsuo Kurabayashi Kenn R Oldham Thomas D Wang |
| |
| Institution: | 1.Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA;2.Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI, USA;3.Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA;4.Department of Electrical Engineering, University of Michigan, Ann Arbor, MI, USA |
| |
| Abstract: | We demonstrate a handheld multiphoton endomicroscope with 3.4 mm distal diameter that can repetitively image mouse colon in vivo. A 2D resonant MEMS mirror was developed to perform beam scanning in a Lissajous pattern. The instrument has an effective numerical aperture of 0.63, lateral and axial resolution of 2.03 and 9.02 μm, respectively, working distance of 60 μm, and image field-of-view of 300 × 300 μm2. Hoechst was injected intravenously in mice to stain cell nuclei. We were able to collect histology-like images in vivo at 5 frames/sec, and distinguish between normal and pre-malignant colonic epithelium.OCIS codes: (170.0170) Medical optics and biotechnology, (060.2350) Fiber optics imaging, (110.0110) Imaging systems, (190.4180) Multiphoton processes, (170.2150) Endoscopic imaging |
| |
| Keywords: | |
|
|
