Ribosomal DNA as molecular markers and their applications in the identification of fish parasites (Platyhelminthes: Monogenea) from India |
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| Affiliation: | 1. Department of Pharmacolgy, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Dhulapally(Post via Hakimpet), Secunderabad (500014 AP, India;2. Department of Pharmacy, Priyadarshini College of Pharmacy, Chowdaryguda Ghatkesar;1. San Raffaele Telethon Institute for Gene Therapy, San Raffaele Scientific Institute, Milan, Italy;2. Vita Salute San Raffaele University, Milan, Italy;3. Current address: Experimental Cancer Genetics, The Wellcome Trust Sanger Institute, Cambridge, UK;4. Current address: Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands;1. Wakayama Research Laboratories of Kao Corporation, 1334 Minato, Wakayama-shi, Wakayama 640-8580, Japan;2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan;1. Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada;2. Departments of Chemistry and Earth, Ocean, & Atmospheric Sciences, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada;3. Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA;4. Department of Oncology, McGill University, Montreal, Quebec H3G 1Y6, Canada;5. Rosalind and Morris Goodman Cancer Research Center, McGill University, Montreal, Quebec H3G 1Y6, Canada |
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| Abstract: | The development of molecular techniques for taxonomic analysis of monogenean parasites has led to a great increase for proper identification and factualness. These molecular techniques, in particular the use of molecular markers, have been used to identify and validate the monogenean parasites. Although, improvements in marker detection systems particularly of elements of rDNA like 18S, ITS and 28S used in monogeneans parasites have enabled great advances to be made in recent years in India. However, the molecular sequence analysis and phylogenetic relationships among the parasitic helminthes is unconventional in India. Many workers have been always questioned the validity of Indian species of monogeneans and emphasized the need to ascertain the status of species from Indian fish. Here we would like to provide additional resolution for the interpretation of use of molecular markers in study of monogeneans in India. This review provides an overview of current stage of studies in India that have been used in applying molecular techniques to monogenean. |
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| Keywords: | Monogenea Ribosomal DNA Molecular markers Fish parasites India |
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