Effect of henna and roselle extracts on pathogenic bacteria |
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| Institution: | 1. Botany Agriculture Department, Faculty of agriculture, South Valley University, Qena, Egypt;2. Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt;1. Research and Graduate Studies in Food Science, Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las campanas s/n, 76010 Querétaro, Qro., Mexico;2. Research and Graduate Studies Division, Dirección General de Innovación Tecnológica, Universidad Autónoma de Tamaulipas, Centro Universitario, 87140 Cd. Victoria, Tam., Mexico;3. Research Group of Functional Foods and Nutraceuticals, Departamento de Ingenierías Química y Bioquímica, Instituto Tecnológico de Durango, Felipe Pescador 1830 Ote., 34080 Durango, Dgo., Mexico;1. Department of Pharmaceutical Sciences, Faculty of Science, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa;2. 339 Tallmadge Rd., Kent, OH 44240, USA;1. Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria;2. Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria;3. Department of Biological Sciences, College of Sciences and Engineering, Landmark University, Omu-Aran, Kwara, Nigeria |
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| Abstract: | ObjectiveTo investigate the antibacterial effects of water and ethanolic extracts of henna leaves and roselle calyxes against pathogenic bacteria isolated from domestic wastewater.MethodsThe antimicrobial activity was determined in the extracts using agar disc diffusion method. The antibacterial activities of extracts (2.5%, 5.0% and 10.0% w/v) of both henna and roselle were tested against one Gram-positive Bacillius subtilis; two Gram-negative Escherichia coli, Pseudomonas aeruginosa human pathogenic bacteria.ResultsEthanolic extracts had more antimicrobial activity than water extracts. Ethanolic extract of roselle had the highest antibacterial activity against all tested organisms, followed with ethanolic extract of henna. Pseudomonas aeruginosa was the most sensitive bacteria to plant extracts.ConclusionThe results of this study suggested that roselle contains more phyto-chemicals with antimicrobial activity than henna on the bacteria strains under study, and these phyto-chemicals were more effective when extracted by ethanol rather than water. |
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| Keywords: | Antimicrobial activity Henna Roselle |
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