Anti-hyperbilirubinemic and wound healing activity of aqueous extract of Calotropis procera leaves in Wistar rats

Aims:

The aim of this study was to evaluate the bilirubin lowering and wound healing property of aqueous extract of Calotropis procera (AECP) leaves in Wistar rats.

Materials and Methods:

Albino Wistar rats of either sex were used for the study. Bilirubin lowering property of C. procera leaves was evaluated using phenylhydrazine and paracetamol as inducing agents followed by measuring the concentration of serum total bilirubin in hyperbilirubinemic rats. Wound healing property was evaluated using incision and excision models by measuring tensile breaking strength, percentage wound contractions, and epithelization days, respectively.

Statistical Analysis:

Statistical comparison between groups in each experiment was done with one-way analysis of variance followed by Dunnett''s test.

Results:

AECP showed a significant (P < 0.05) decrease in concentrations of serum total bilirubin in hyperbilirubinemic rats as well as significant (P < 0.05) increase in breaking strength and percentage wound contractions with decreased epithelization period when compared to control groups.

Conclusions:

AECP showed significant bilirubin lowering and wound healing property in Wistar rats.KEY WORDS: Calotropis procera, excision, hyperbilirubinemia, incision, paracetamol, phenylhydrazine, wound healing