Establishment and identification of human primary lung cancer cell culture in vitro
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Authors: | Lian-Lian Si Lu Lv Wen-Hui Zhou Wei-Dong Hu |
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Institution: | 1.Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430071, China;2.Hubei Cancer Clinical Study Center & Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan, Hubei 430071, China;3.Department of People’s Hospital of Beijing Daxing District, Beijing 102600, China;4.Medical Center for Human Reproduction, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China |
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Abstract: | Objective: To explore a simple and practical method for human primary lung cancer cells culture in vitro. Methods: Tumor specimens from 6 lung cancer patients were isolated with collagenase digestion cultured in vitro. Then the characteristics of these cells were analyzed and identified by optical microscope observation, hematoxylin-eosin staining, immunocytochemistry, immunohistochemistry and tumor nude mice inoculation experiments, respectively. Results: Except for the small cell lung cancer, the other 5 samples were successfully isolated and cultured. The cultured cells showed typical characteristics of malignant cells and positive for cytokeratin 7 and 19. Moreover, the cancer cells readily formed subcutaneous tumors in nude mice and the pathological images of the transplanted tumor were consistent with its tumor origin. Conclusion: The primary culture for human lung cancer cells can be successfully achieved with the method of collagenase digestion. |
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Keywords: | Lung cancer primary cell culture morphology H-E staining immunohistochemistry |
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