Characterization of novel non-protein-coding RNA from V. cholerae O1 El Tor |
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| Institution: | 1. Advanced Medical and Dental Institute, University Sains Malaysia, Bertam13200 Kepala Batas, Malaysia;2. Institute fur Experimentelle Pathologie, Unversitat Munster, D48149 Munster, Germany;1. Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland;2. deepCDR Biologics AG, Basel, Switzerland;3. Botnar Research Centre for Child Health, Basel, Switzerland;4. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden;5. Institute of Microbiology and Immunology, Department of Biology, ETH Zurich, Zurich, Switzerland;6. Department of Pathology and Immunology, University of Geneva, Geneva, Switzerland;7. Swiss Institute of Bioinformatics, Mattenstr. 26, 4058 Basel, Switzerland;8. Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland;9. Department of Surgery, Oral and Cranio-Maxillofacial Surgery, University Hospital Basel, Basel, Switzerland;10. Department of Health, Economics and Health Directorate, Canton Basel-Landschaft, Switzerland;1. Internal Medicine Department, Faculty of Medicine, Ain Shams University, Egypt;2. Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Egypt;1. Biocolloid and Fluid Physics Group, Department of Applied Physics, Faculty of Sciences, University of Granada, 18071 Granada, Spain;2. Natural Bioactive Compounds Group, Instituto de Biología Molecular y Celular (IBMC), Universidad Miguel Hernández, Elche, Alicante, Spain;1. School of Chemistry, University of Sydney, NSW 2006, Australia;2. School of Chemistry, University of Melbourne, Victoria 3000, Australia;3. Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia;4. Institut für Nukleare Entsorgung, Karlsruher Institut für Technologie (KIT), Herrmann-von-Helmholtz-Platz 1, 76433 Eggenstein-Leopoldshafen, Germany;1. State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510640, China;2. Institute of Polymer Science, DSAPM Lab, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China;3. School of Media and Communication, Shenzhen Polytechnic, Shenzhen 518055, China |
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| Abstract: | IntroductionA plethora of bacterial small RNA (npcRNA) has progressively gained momentum in revising our apprehension on their engagement as key player in modulating general stress responses and bacterial virulence. Vibrio cholerae serogroup O1, biotype El Tor causes cholera, which is an acute dehydrating, watery diarrhoeal disease, with epidemic and pandemic potential. We have identified a total of 224 novel npcRNA candidates from V. cholerae El Tor by experimental RNomics strategy. Objective: To characterize the expression profile and functionality of identified novel npcRNA from V. cholerae O1 El Tor.MethodsDifferential expression of selected npcRNA candidates was studied using Northern blot analysis under various stress conditions in the background wild type strain as well as Hfq protein knockout strain. Interaction of the selected npcRNA candidates with Hfq protein was also evaluated.Results & DiscussionTwo intergenic npcRNA genes were incorporated as targets in a multiplex PCR that could be a crucial tool in molecular epidemiological study of V. cholerae. Differential expression of 9 selected npcRNA candidates under a series of stress conditions was demonstrated. Interestingly, one of the candidate, Vc_npcR_3853 was demonstrated to have the potential in negatively regulating the expression of VC0092 and VC0304, which encodeLexA repressor and guanosine-5′-triphosphate, 3′-diphosphate pyrophosphatase, respectively.ConclusionCollectively, differential expression of those npcRNA candidates under wild type and Hfq knockout background as well as their interaction with Hfq protein set the stage towards discerning the functional role of these novel npcRNAs in orchestrating pathophysiology of V. cholerae El Tor. |
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