In vitro antitrypanosomal activity,antioxidant property and phytochemical constituents of aqueous extracts of nine Nigerian medicinal plants |
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| Affiliation: | 1. Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Biológicas, Laboratorio de Patología Molecular, Ave. Universidad S/N, Cd. Universitaria, 66451 San Nicolás de los Garza, NL, Mexico;2. Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Biológicas, Laboratorio de Fitoquímica, Ave. Universidad S/N, Cd. Universitaria, 66451 San Nicolás de los Garza, NL, Mexico;1. Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria;2. Department of Biochemistry, Umaru Musa Yaradua University, Katsina, Nigeria;3. Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria;1. Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo;2. Centre for Research and Conservation/KMDA, Koningin Astridplein 20−26, B-2018 Antwerp, Belgium;3. Faculty of Biology/ Department Biology II, Ludwig Maximilian University of Munich,Großhaderner Straße 2, d-82152 Planegg-Martinsried, Germany;4. Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium;5. Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Antwerp, Belgium |
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| Abstract: | ObjectiveTo study the in vitro antitrypanosomal activity, antioxidant property and phytochemical constituents of aqueous extracts of nine Nigerian medicinal plants.MethodsIn vitro antitrypanosomal activity test was carried out on aqueous extracts of dried leaves of Acacia albida (A. albida), Artemisia absinthium, Bryophyllum pinnatum, Gongronema latifolium, Holarrhena floribunda, Leptadenia hastata, Pericopsis laxiflora (P. laxiflora) and dried stem barks of A. albida and P. laxiflora. The phytochemical constituents and composition of the extracts and the in vitro antioxidant activity of the extracts were subsequently measured using the α,α-diphenyl-β-picryl-hydrazyl (DPPH) radical scavenging assay, Ferric reducing antioxidant power (FRAP) assay, thiobarbituric acid (TBA) activity assay and H2O2 radical scavenging activity assay.ResultsFrom the study, it was discovered that the stem bark extracts of A. albida and P. laxiflora were most active against both Trypanosoma evansi and Trypanosoma congolense. There was complete cessation of motility in both trypanosomes within 5 min at 40 mg/mL of the stem bark extract of A. albida and complete cessation of motility within 25 min and 40 min at 40 mg/mL with P. laxiflora stem bark extract for Trypanosoma congolense and Trypanosoma evansi, respectively. Quantitative analysis of the phytochemical constituents of the aqueous extracts of the plant parts such as alkaloids, saponins, flavonoids and phenols revealed that the stem barks of A. albida, P. laxiflora and leaves of Leptadenia hastata contained relatively high amount of all the phytochemicals quantified. The stem bark extracts of A. albida, P. laxiflora and leaves of Gongronema latifolium possess more scavenging capacity when compared to other extracts in relation to vitamin C, the reference antioxidant.ConclusionsThis study provides scientific evidence for the use of A. albida, and P. laxiflora for the treatment of trypanosomosis and diseases associated with oxidative stress. |
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| Keywords: | Aqueous Trypanosomiasis Oxidants Phytochemicals Medicinal plants |
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