HPLC法同时测定复方鼻膏中2组分的含量

目的:建立以高效液相色谱法同时测定复方鼻膏中磺胺嘧啶和醋酸地塞米松含量的方法。方法:色谱柱为Inertsil C8-3,流动相为甲醇-乙腈-水(72∶3∶25),流速为1.0mL·min-1,柱温为40℃,检测波长为240nm,进样量:20μL。结果:磺胺嘧啶和醋酸地塞米松检测浓度的线性范围分别为74.65～746.5(r=0.999 9)、1.495～14.95μg·mL-1(r=0.999 7);平均加样回收率分别为100.72%(RSD=1.7%)、100.41%(RSD=1.6%)。结论:本方法简便易行、准确可靠,可用于该制剂的含量测定。

Simultaneous Determination of Sulfadiazine and Dexamethasone Acetate in Compound Nasal Ointment by HPLC

OBJECTIVE： To establish an HPLC method for simultaneous determination of the content of sulfadiazine and dexamethasone acetate in compound nasal ointment. METHODS： The determination was performed on Inertsil Cs a column with column temperature set at 40 ℃ ; the mobile phase consisted of methanol acetonitrile - water （72 ： 3 ： 25） with a flow rate of 1.0 mL ·min^-1. The detective wavelength was set at 240 nm and the sample size was 20 μL. RESULTS： The linear ranges for sulfadiazine and dexamethasone acetate were 74.65- 746.5（ r = 0. 999 9） and 1. 495- 14.95 μg·mL^ -1（ r = 0. 999 7）, respectively. The average recoveries were 100.72% （RSD =1.7% ） and 100.41% （RSD = 1.6% ）, respectively. CONCLUSION： This method is rapid, simple, accurate and reliable and suitable for the determination of the content of sulfadiazine and dexamethasone acetate in compound nasal ointment.