嗜肺军团菌血清I型菌株随机扩增多态性DNA指纹图谱研究

目的采用随机扩增多态性DNA(RAPD)方法研究我国58株嗜肺军团菌血清1型(Lp1)菌株的基因型特征。方法58株嗜肺军团菌血清1型菌株来自北京和深圳市的集中空调冷却塔水和温泉水,随机引物采用5’-CGGCGGCG-GCGG-3’序列,分析Lp1型军团菌RAPD基因型和温度、pH值的关系。结果58株Lp1型菌株共分为17个RAPD基因型。与集中空调冷却塔水Lp1型菌株相比,温泉水分离的菌株基因型具有较高的多态性。深圳市29株集中空调冷却塔水分离的Lp1菌株,共呈现出3个RAPD基因型,北京市24株来自温泉水的Lp1菌株分为12个RAPD型,5株来自集中空调冷却塔水的Lp1型菌株分为两个RAPD型。温泉水Lp1菌株的基因型差异和水温度和pH值无明显的关联。结论RAPD分型方法可用于我国Lp1菌株的基因分型,不同地区和来源的Lp1型军团菌菌株具有特征性的RAPD基因型,温泉水中分离的Lp1菌株较集中空调冷却塔水分离菌株基因型呈现较高的基因多态性。

DNA fingerprinting of Legionella pneumophila serogroup 1 strains by Randomly Amplified Polymorphic DNA analysis

58 Legionella pneumophila serogroup 1 (Lp1) strains, isolated from cooling towers and spa pools from Beijing and Shenzhen cities, were analyzed by Randomly Amplified Polymorphic DNA analysis technique. The nucleotide sequence SK2 CGGCGGCGGCGG (5'-3') was used as random primer for RAPD analysis. Temperatures and pH of the original water systems were also analyzed of different RAPD patterns. 58 Legionella pneumophila serogroup 1 strains were divided into 17 RAPD patterns, especially the strains from spa pools showing highly discriminatory polymorphism. For the strains isolated from cooling towers, 3 RAPD patterns with 29 isolates were found in Shenzhen and 2 RAPD patterns with 5 isolates in Beijing. 24 strains isolated from spa pools in Beijing showed 12 discriminatory patterns. No evidence showed correlation between the RAPD patterns and the temperatures and pH of the water. RAPD techniques proved to be as a genotyping method for Lp1 strains analysis. Strains from different areas and original water systems in China showed different characteristic RAPD patterns, especially strains from the spa pools with highly discriminatory polymorphisms than strains from cooling towers.