麻杏石甘汤及其加味对A型流感病毒感染小鼠T细胞亚群的影响

目的研究麻杏石甘汤及其加味对A型流感病毒感染小鼠T细胞亚群的影响。方法用甲型流感病毒鼠肺适应株经鼻腔接种小鼠,建立感染模型。实验设10倍麻杏石甘汤组、5倍麻杏石甘汤组、感染模型组、正常对照组。正常对照组、感染模型组灌胃给生理盐水,其它各组灌胃给相应浓度的药物,1次/d,0.4 ml/次,连续8 d。最后一次给药后禁水、禁食8 h,取标本进行T细胞亚群百分率的检测。结果10倍麻杏石甘汤加味方组CD3 、CD4 T细胞亚群百分率、CD4 /CD8 比值显著高于感染模型组(P<0.05),CD8 T细胞亚群百分率显著低于感染模型组(P<0.05)。10倍麻杏石甘汤组、5倍麻杏石甘汤加味方组CD8 T细胞亚群百分率显著低于感染模型组(P<0.05)。结论10倍临床最大用量的麻杏石甘汤和5倍临床最大用量的麻杏石甘汤加味方能明显调整流感病毒感染小鼠T细胞亚群的百分率。

Effect of Maxin Shigan Decoction and its Alteration on Mice T - cell Subpopulation Exposured to Influenza Virus A

Objective To investigate the effect of maxin shigan decoction(MSD) and its alteration on mice T-cell subpopulation exposed to influenza virus A.Methods The models were established by inoculating with the influenza virus A fitted to lung via mice nose in mice and then divided into 10 times of MSD,5 times of MSD,infected model and normal control groups.The normal salt solution was filled up in the control and infected groups and the drug MSD in relevant concentration in the other 2 groups,1 time per day,each time with 0.4ml,and lasted for 8 days. The water or drug was forbidden for 8 hours after the last time of filling up,and then,the percentage of T-cell subpopulation was measured from the experimental animals.Results The percentages of CD3 and CD4 T-cell subpopulation and the ratio of CD4 /CD8 in the 10 times MSD group were significantly higher than those in the infected model group(P<0.05) while the percentage of CD8 T-cell subpopulation was significantly lower than that of the infected model group(P<0.05).The percentages of CD8 T-cell subpopulation in both 10 and 5 times of MSD groups were significantly lower than those of the infected model group(P<0.05).Conclusion MSD in the largest dosage(10 times of clinical dose) and supplemented MSD in the largest dosage(5 times of clinical dose) can obviously regulate the percentage of T-cell subpopulation in mice exposed to influenza virus A.