臭氧预处理对体外循环伴心脏停搏大鼠肾脏损伤的影响

目的 探讨臭氧预处理对体外循环伴心脏停搏大鼠肾脏损伤的保护作用.方法 健康清洁级雄性SD大鼠24只随机分为假手术组（SH组）、体外循环组（CPB组）、臭氧预处理组（OP组）,每组8只.SH组只做外科操作不做体外循环,OP组手术前腹腔注射臭氧300 μg/kg,浓度50 μg/ml,每天1次,连续5d.CPB组每天注射相同体积的氧气.CPB组和OP组通过微型化体外循环环路设备,经右颈静脉腔房引流、右股动脉灌注方法建立无血预充大鼠CPB伴心脏停搏模型,体外循环停止即刻取右股动脉血2ml后处死,观察大鼠血浆肌酐（creatinine,Cr）、肿瘤坏死因子α（tumor necrosis factor-alpha,TNF-α）、白细胞介素-6（interleukin-6,IL-6）、IL-10、肾脏组织8-异-前列腺素Fzα（8-iso-PGFbzα）水平、超氧化物歧化酶（superoxide dismutase,SOD）括性,并观察肾脏组织超微结构改变.结果 与SH组比较,CPB组和OP组血清肌酐、TNF-α、IL-6含量增高（P=0.000）,肾脏组织8-iso-PGF2α水平升高（P=0.000）,血浆IL-10水平、肾脏组织SOD活性降低（P =0.000）；与CPB组比较,OP组血清肌酐、TNF-α、IL-6含量降低（P=0.000）,肾脏组织8-iso-PGF2α水平降低（P=0.000）,血浆IL-10水平、肾脏组织SOD活性增高（P=0.000）；与CPB组比较,OP组病理损伤程度减轻.结论 臭氧预处理可降低脂质过氧化水平,减轻炎症反应程度,减轻体外循环伴心脏停搏大鼠肾脏损伤程度.

Effect of ozone oxidative preconditioning in rat renal injury following cardiopulmonary bypass and cardioplegic arrest

Objective To analyze the protective effect of ozone oxidative preconditioning on renal injury in rats after cardiopulmonary bypass and cardioplegie arrest. Methods 24 male SD rats of clean grade were randomly divided into sham-operated group（ SH group） ,cardiopulmonary bypass group（ CPB group） and ozone preconditioning group（ OP group） ,and each group included 8 rats. The SH group： the surgery were performed in the rats without CPB. The OP group： the fresh oxygen/ozone gas mixture （300 μg/kg, ozone concentration of 50 μg/ml） were intraperitoneal injected in the left lower abdomen of rats, once a day （8：30 AM ） for 5 days preoperatively. The CPB group：the fresh oxygen gas were intraperitoneal injected in the left lower abdomen of rats, once a day for 5 days preoperatively. CPB with CA Rats models were established by the right jugular vein drainage and right femoral artery perfusion using the mini CPB loop circuit equipment in both CPB group and OP group. The 2 ml blood from right femoral artery was acquired immediately after CPB was stopped, and then the rats were killed. The indicators of assessment included serum creatinine levels, tumor necrosis factor-alpha, interleukin-6, interleukin-10, 8-iso-PGF2α levels and SOD activity of renal tissue,and the ultra microstructure of renal tissue. Results Serum ereatinine,TNF- α,IL-6 levels and 8-iso-PGF2α levels of renal tissue at each time point of CPB group and OP group significantly increased in comparation with those of SH group, and the difference was statistically significant （ P = 0. 000 ） ; However, serum IL-10 levels, SOD activity of renal tissue at each time point of CPB group and OP group significantly decreased in comparation with those of SH group,and the difference was statistically significant（P =0. 000）. Serum creatinine,TNF-α, IL-6 levels and 8-iso-PGF2α levels of renal tissue at each time point of OP group significantly decreased in eomparation with those of CPB group, and the difference was statistically significant （ P = 0. 000 ） ; However, serum IL-10 levels, SOD activity of renal tissue at each time point of CPB group and OP group significantly increased in comparation with those of CPB group, and the difference was statistically significant （ P = 0. 000 ）. The pathological injuries for renal ultrastructure in OP group were smaller than those of renal ultrastructure in CPB group. Conclusion Ozone preconditioning can reduce the levels of the lipid peroxidation, decrease inflammatory response and relieve the renal injury in rats following cardiopulmonary bypass and cardioplegic arrest.