Analysis of intrahepatic HBVDNA in serologic HBV markers-negative or HBsAg-negative patients with chronic hepatitis |
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Authors: | Zhang Yong-yuan Yu Zi-qun Hao Lian-jie |
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Institution: | (1) Clinical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wulian |
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Abstract: | Summary To explore HBV infection status in a group of serologic HBV markers-negative or HBsAg-negative patients with chronic hepatitis,
in situ hybridization to hepatccellular HBVDNA was carried out in combination with detection of HBsAg and HBcAg in the liver
tissues. It was found that prevalence of intrahepatic HBVDNA, HBsAg and HBcAg was 43 %, 39 % and 17 % respectively, and 15
out of 23 cases studied were sure to bear one or more positive marker (s) of intrahepatic HBVDNA, HBsAg and HBcAg. These findings
suggest that more than half of the patients with chronic hepatitis were still undergoing HBV infection, despite serologic
HBV markers or HBsAg negative. Furthermore, we found that hepatocytes containing HBV-DNA or surface or core antigen were often
close to hepatic necrosis foci, indicating that HBV replication and its antigen(s) expression in hepatocytes could account
for chronic, active and necrotic inflammation occurring in the liver of the patients mentioned above. |
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Keywords: | chronic hepatitis HBV markers HBVDNA in situ hybridization pathogenesis |
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