RP-HPLC法同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量

目的:建立RP-HPLC法同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量。方法:采用AgilentExtent-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水(47∶53)为流动相,流速为1.0 mL.min-1,柱温为35℃,检测波长为225 nm。结果:厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的浓度分别在2.744～137.2μg.mL-1(r=0.9999),2.836～141.8μg.mL-1(r=0.9999),3.164～158.2μg.mL-1(r=0.9998)及3.148～157.4μg.mL-1(r=0.9999)范围内与峰面积线性关系良好;方法回收率(n=3)为96.3%～102.7%,RSD为0.2%～1.7%。结论:本方法简便、准确,重复性好,可用于同时测定保济丸中厚朴酚、和厚朴酚、木香烃内酯及去氢木香内酯的含量。

RP - HPLC simultaneous determination of magnolol,honokiol,costunolide and dehydrocostuslactone in Baoji pills

Objective:To develop an RP-HPLC method for the simultaneous determination of magnolol,honokiol,costunolide and dehydrocostuslactone in Baoji pills.Methods:The analysis was carried out on an Agilent Extent-C18(250 mm×4.6 mm,5 μm)column;The mobile phase was acetonitrile-water(47∶53)and the flow rate was 1.0 mL·min-1;The column temperature was 35 ℃ and the detection wavelength was set at 225 nm.Results:The linear ranges of magnolol,honokiol,costunolide and dehydrocostuslactone were 2.744-137.2 μg·mL-1(r=0.9999),2.836-141.8 μg·mL-1(r=0.9999),3.164-158.2 μg·mL-1(r=0.9998)and 3.148-157.4 μg·mL-1(r=0.9999),respectively.The average recovery(n=3)was 96.3%-102.7% and RSD was 0.1%-1.7%.Conclusion:The method is simple,and can be used for determination of the four components in Baoji Pills with satisfactory accuracy and repeatability.