Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker |
| |
Authors: | Hoon Park Nathan I Lopez Alan T Bakalinsky |
| |
Institution: | (1) Department of Food Science and Technology, Wiegand Hall, Oregon State University, Corvallis, OR 97331-6602, USA e-mail: bakalina@bcc.orst.edu Tel.: +1-541-737-6510; Fax: +1-541-737 1877, US |
| |
Abstract: | Two S. cerevisiae genes were found to exhibit dominant phenotypes useful for selecting transformants of industrial and laboratory strains of
S. cerevisiae. FZF1-4, which confers sulfite resistance, was originally isolated and identified as RSU1-4, but the two genes are shown here to be allelic. Cysteine 57 in wild-type Fzf1p was found to be replaced by tyrosine in Fzf1-4p.
Multicopy SSU1, which also confers sulfite resistance, was found to be somewhat less efficient. In both cases, a period of outgrowth in
non-selective medium following transformation was found to be necessary. The number of transformants obtained was found to
be strain-dependent, and also to depend on the sulfite concentration used during selection. Undesirable background growth
of non-transformants was not observed at cell densities as high as 2.5 × 107/plate. In two ura3 laboratory strains where selection for URA3 was applied independently of that for sulfite, the transformation efficiency for sulfite resistance was about 50% that for
uracil prototrophy.
Received: 1 July / 9 August 1999 |
| |
Keywords: | Transformation Sulfite Brewing Wine Baking Industrial yeast S cerevisiae Yeast Sulfur dioxide Selenite |
本文献已被 PubMed SpringerLink 等数据库收录! |
|