| 人脂肪间充质干细胞体外定向诱导分化为胰岛样细胞的实验研究 |
| |
| 引用本文： | 武晓佳,任刚,边云飞,何军华,肖传实.人脂肪间充质干细胞体外定向诱导分化为胰岛样细胞的实验研究[J].中国药物与临床,2010,10(4):394-396,I0001. |
| |
| 作者姓名： | 武晓佳 任刚 边云飞 何军华 肖传实 |
| |
| 作者单位： | 山西医科大学第二医院心内科,太原,030001 |
| |
| 摘 要： | 目的体外定向诱导人脂肪间充质干细胞向胰岛样细胞的分化。方法人脂肪间充质干细胞分3个阶段进行诱导,第一阶段培养在含适当浓度的2-巯基乙醇的高糖-达氏修正依氏培养基(HG-DMEM)培养2d,第二阶段在含适当浓度的B27、碱性成纤维细胞生长因子(bFGF)及表皮生长因子(EGF)的HG-DMEM培养基中诱导6d,第三阶段在含适当浓度的2-巯基乙醇和B27和尼克酰胺高糖无血清DMEM培养基诱导细胞向胰岛样细胞分化。对照组用HG-DMEM培养。在相差显微镜下观察细胞的形态;用反转录-聚合酶链反应(RT-PCR)法检测诱导前后nestin、胰岛素基因的表达;用免疫荧光染色法检测诱导前后nestin、胰岛素的表达;诱导第三阶段进行双硫腙染色鉴定胰岛B样细胞团。结果未经诱导的脂肪间充质干细胞呈长梭形贴壁生长,诱导后细胞逐渐变圆,并聚集成团。诱导8d细胞nestin基因呈阳性表达,诱导14d细胞nestin基因表达量下降,胰岛素基因表达呈阳性。诱导后的细胞团双硫腙染色呈棕红色。结论2-巯基乙醇、EGF、bFGF及尼克酰胺等可在体外诱导人脂肪间充质干细胞分化为具有分泌胰岛素功能的胰岛样细胞。
|
| 关 键 词： | 间质干细胞 细胞分化 胰岛样细胞 |
In vitro differentiation of adult adipose-derived mesenchymal stem cells into islet-like cells |
| |
| WU Xiao-jia,REN Gang,BIAN Yun-fei,HE Jun-hua,XIAO Chuan-shi.In vitro differentiation of adult adipose-derived mesenchymal stem cells into islet-like cells[J].Chinese Remedies & Clinics,2010,10(4):394-396,I0001. |
| |
| Authors: | WU Xiao-jia REN Gang BIAN Yun-fei HE Jun-hua XIAO Chuan-shi |
| |
| Institution: | WU Xiao-jia,REN Gang,BIAN Yun-fei,HE Jun-hua,XIAO Chuan-shi.Department of Cardiology,Second Hospital of Shanxi Medical University Taiyuan 030001,China |
| |
| Abstract: | Objective To study the process for in vitro differentiation of adult adipose-derived mesenchymal stem cells (ADMSCs) into islet-like cells.Methods Induced differentiation of the ADMSCs into islet-like cells was performed in three development stages-incubation in HG-DMEM supplemented with 2-mercaptoethanol for 2 days (stage 1),incubation in HG-DMEM supplemented with basic fibroblast growth factor (bFGF),epidermal growth factor (EGF) and B27 for 6 days (stage 2),and incubation in FBS-free HG-DMEM supplemented... |
| |
| Keywords: | Mesenchymal stem cells Cell differentiation Islet-like cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
